| 分割照射对肝癌移植瘤小鼠脾脏免疫细胞的影响 |
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| 引用本文： | 姚丹,张俊玲,郑旺,陈倩萍,胡松玲,潘燕,邵春林.分割照射对肝癌移植瘤小鼠脾脏免疫细胞的影响[J].中华放射医学与防护杂志,2020,40(2):82-87. |
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| 作者姓名： | 姚丹 张俊玲 郑旺 陈倩萍 胡松玲 潘燕 邵春林 |
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| 作者单位： | 复旦大学放射医学研究所, 上海 200032,复旦大学放射医学研究所, 上海 200032,复旦大学放射医学研究所, 上海 200032,复旦大学放射医学研究所, 上海 200032,复旦大学放射医学研究所, 上海 200032,复旦大学放射医学研究所, 上海 200032,复旦大学放射医学研究所, 上海 200032 |
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| 基金项目： | 国家重点研发计划项目（2017YFC0108604）；国家自然科学基金（31770910，31570850） |
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| 摘 要： | 目的探讨肝癌皮下移植瘤小鼠的分割照射对远端脾脏组织的影响。方法构建荷瘤C57BL/6 J小鼠模型,取对数期生长的小鼠肝癌细胞Hepa 1-6接种于小鼠右侧皮下(1×10^7/只),荷瘤小鼠按随机数表法分为荷瘤对照组和照射组,每组20只。另设10只健康小鼠作为健康对照组。照射组皮下肿瘤给予8 Gy×3次X射线局部照射,剂量率为0.883 Gy/min。照射后第7、14天检测小鼠肿瘤脏器指数、脾脏脏器指数、脾脏病理学改变,以及脾脏T淋巴细胞亚群、B淋巴细胞亚群、NK细胞的变化。结果与荷瘤对照组相比,照后7、14 d,照射组小鼠肿瘤脏器指数显著降低(t=4.649、26.34,P<0.05),脾脏中NK细胞显著升高(t=3.952、3.633,P<0.05),CD3+、CD4+、CD3+CD4+淋巴细胞以及CD4+/CD8+比值在照后7 d显著降低(t=3.193、3.656、3.219、2.641,P<0.05),CD3+淋巴细胞在照后14 d显著降低(t=3.031,P<0.05),其余指标脾脏脏器指数、B淋巴细胞、CD3+CD4+淋巴细胞、CD8+淋巴细胞变化不显著。结论肿瘤局部照射可引起远隔器官内淋巴细胞比例失衡,导致机体免疫力下降,为放疗免疫损伤提供了新的诠释。
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| 关 键 词： | 肝癌 分割照射 脾脏淋巴细胞 |
| 收稿时间： | 2019/8/29 0:00:00 |
Effect of fractionated radiotherapy of transplanted hepatocellular carcinoma on the splenic immune cells in mice |
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| Yao Dan,Zhang Junling,Zheng Wang,Chen Qianping,Hu Songling,Pan Yan and Shao Chunlin.Effect of fractionated radiotherapy of transplanted hepatocellular carcinoma on the splenic immune cells in mice[J].Chinese Journal of Radiological Medicine and Protection,2020,40(2):82-87. |
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| Authors: | Yao Dan Zhang Junling Zheng Wang Chen Qianping Hu Songling Pan Yan and Shao Chunlin |
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| Affiliation: | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China,Institute of Radiation Medicine, Fudan University, Shanghai 200032, China,Institute of Radiation Medicine, Fudan University, Shanghai 200032, China,Institute of Radiation Medicine, Fudan University, Shanghai 200032, China,Institute of Radiation Medicine, Fudan University, Shanghai 200032, China,Institute of Radiation Medicine, Fudan University, Shanghai 200032, China and Institute of Radiation Medicine, Fudan University, Shanghai 200032, China |
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| Abstract: | Objective To investigate the effect of fractionated radiotherapy on the immune system of mice with subcutaneously transplanted hepatocellular carcinoma. Methods Logarithmic growth of mouse hepatocellular carcinoma cells Hepa 1-6 were inoculated subcutaneously on the right side of C57BL/6 J mice (1×107 cells/mice). The tumor-bearing mice were randomly divided into control group (Ctrl) and irradiation group (IR), 20 mice in each group. Additionally, 10 healthy mice were set as normal control group. Local fractionated X-ray irradiation of 8 Gy×3 fraction was given to the subcutaneous tumors, and the dose rate was 0.883 Gy/min. At 7 and 14 d after irradiation, the tumor organ index, spleen organ index, spleen pathological changes, and splenic T lymphocyte subsets, B lymphocyte subsets, and NK cells were detected. Results Compared with Ctrl, at 7 and 14 d after irradiation, the tumor organ index decreased (t=4.649, 26.34,P<0.05), and the percentage of NK cells increased significantly (t=3.952, 3.633,P<0.05). The percentages of CD3+, CD4+, CD3+CD4+ lymphocytes and the ratio of CD4+/CD8+ lymphocyte decreased at 7 d after irradiation (t=3.193, 3.656, 3.219, 2.641,P<0.05), and the percentage of CD3+lymphocyte decreased at 14 d after irradiation (t=3.031,P<0.05). But after irradiation, there were no significant changes in spleen organ index, B lymphocyte, CD3+ CD4+ lymphocyte and CD8+ lymphocyte. Conclusions Local hepatoma radiotherapy causes imbalance of lymphocytes in distal spleen of mice and hence reduces immunity, which provides a novel mechanism of radiological immunity damage. |
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| Keywords: | Hepatocellular carcinoma Fractionated radiotherapy Splenic lymphocytes |
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