放射或放射联合泰素对鼻咽癌细胞p57kip2蛋白表达的影响

目的　研究放射或放射联合泰素对鼻咽癌细胞抑癌基因p5 7kip2 蛋白表达的影响。方法　采用免疫组织化学法和WesternBlot法检测鼻咽癌细胞照射前后p5 7kip2 蛋白表达情况。结果p5 7kip2 蛋白在鼻咽癌细胞中弱阳性表达 ,单纯照射组和照射联合泰素组可见p5 7kip2 蛋白在照射后随吸收剂量增加和时间延长其表达均上调 (P <0 0 1) ,照射联合泰素组的p5 7kip2 蛋白表达比单纯照射组明显增强 (P <0 0 1)。结论　放射或放射联合泰素对鼻咽癌细胞抑癌基因p5 7kip2 蛋白表达有明显的增强作用

Effect of irradiation or irradiation combined with taxol on expression of antiongene p57Kip2 in nasopharyngeal carcinoma cells in vitro

Objective\ To study the effect of irradiation or irradiaton combined with taxol on expression of antioncogene p57~(kip2) in nasopharyngeal carcinoma cells. Methods\ To test the expression of antioncogene p57~(kip2) by immunohistochemistry and Western blot before and after irradiation of the cells. Results\ The expression of p57~(kip2) protein was weak in nasopharyngeal carcinoma cells.Both in single irradiation group and in irradiation combined with taxol group,the expression of P57~(kip2) protein improved along with increase of dose and time length after irradiation(P<0.01).The expression of P57~(kip2) protein was stronger in the irradiation combined with taxol group than in the single irradiation group(P<0.01). Conclusion\ The up-regulation of expression of p57~(kip2) antioncogene may reflect the enhancement of radiosensitivity of the nasopharyngeal carcinoma cells induced by both single irradiation or irradiation combined with taxol.