榄香烯对小鼠脾淋巴细胞亚群及其调节的辐射保护效应

探讨榄香烯对受照小鼠脾淋巴细胞及亚群间调节作用的保护效应和剂量范围。方法应用单克隆抗体铺皿法分离受６０Ｃｏγ射线分次照射的小鼠脾脏Ｌ３，Ｔ４，Ｌｙｔ２和Ｂ细胞，分别测定榄香烯对以上三种亚群细胞和Ｌ３，Ｔ４，Ｌｙｔ２对Ｂ细胞３Ｈ－ＴｄＲ掺入的影响，同时测定ＳＯＤ活力和ＬＰＯ含量。结果榄香烯提高了受照２．５Ｇｙ（０．５Ｇｙ×５ｄ）小鼠脾淋巴细胞亚群的辐射抗性，增强了Ｌ３Ｔ４对Ｂ细胞的辅助调节作用，提高了脾脏ＳＯＤ活力和降低ＬＰＯ含量。而对受照４Ｇｙ（０．８Ｇｙ×５ｄ）和５Ｇｙ（０．５Ｇｙ×１０ｄ）组没有观察到这种效应。结论在一定剂量范围，榄香烯通过清除自由基减少靶分子的损伤。

Radioprotective effects of elemene on spleen lymphocyte subsets of mice and its regulation.

Objective To explore the radioprotective effcets of elemene on spleen lymphocyte subsets of mice and the relationship between dose and effect, and its regulation.Methods The spleen lymphocytes in mice were separated into L₃T₄, Lyt₂ and B cells after fractional irradiation by a panning method with monoclonal antibodies, and the effects of elemene on function and regulation of lymphocyte subsets were studied by using 3 H TdR incorporation. The SOD activety and LPO content were measured as well.Results Elemene could increase the radiation resistance of lymphocyte subsets of 2.5Gy(0.5Gy×5d) group, enhance the auxiliary effects of L₃T₄ on B cell, increase spleen SOD activity and decrease LPO content. Not such effect were observed in 4Gy(0.8Gy×5d) and 5Gy(0.5Gy×10d) gyoups.Conclusion The mechanism of protection of elemene in limied dose range may be due to its scavenging free radicals and relieving the damage of target cells.