| 131Ⅰ-抗-c-erbB-2单抗对卵巢癌细胞SKOV3的抑制作用 |
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| 引用本文： | 张丙忠,张莘,王静,蒋宁一,沙孝珍,林仲秋.131Ⅰ-抗-c-erbB-2单抗对卵巢癌细胞SKOV3的抑制作用[J].中华核医学杂志,2005,25(1):41-42. |
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| 作者姓名： | 张丙忠 张莘 王静 蒋宁一 沙孝珍 林仲秋 |
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| 作者单位： | 1. 510120,广州,中山大学附属第二医院妇产科
2. 510120,广州,中山大学附属第二医院核医学科 |
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| 基金项目： | 广东省科委攻关基金资助项目(A000099029) |
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| 摘 要： | 目的观察^131Ⅰ标记针对癌蛋白p185为靶点的单克隆抗体在体外对卵巢癌细胞的抑制作用。方法用氯胺T法^131Ⅰ标记抗-c-erbB-2单克隆抗体(anti-c-erbB-2-McAb)，取不同浓度的^131Ⅰ-anti-c-erbB-2-McAb与SKOV3细胞共同培养，以流式细胞术检测其对SKOV3细胞生长的抑制作用。结果^131Ⅰ-anti-c-erbB-2-McAb在体外可明显抑制SKOV3细胞，^131Ⅰ-rmIgG的抑制作用较弱，未标记的anti-c-erbB-2-McAb对SKOV3细胞的生长几乎没有抑制作用：结论^131Ⅰ-anti-c-erbB-2-McAb能有效抑制肿瘤细胞生长，有可能用于卵巢癌的免疫导向治疗。
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| 关 键 词： | ^131 SKOV3 c-erbB-2 抑制作用 抗-c 卵巢癌细胞 体外 p185 细胞生长 |
The inhibitive effect of 131I- anti-c-erbB-2-McAb on ovarian carcinoma |
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| ZHANG Bing-zhong,ZHANG Xin,WANG Jing,et al..The inhibitive effect of 131I- anti-c-erbB-2-McAb on ovarian carcinoma[J].Chinese Journal of Nuclear Medicine,2005,25(1):41-42. |
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| Authors: | ZHANG Bing-zhong ZHANG Xin WANG Jing |
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| Affiliation: | ZHANG Bing-zhong,ZHANG Xin,WANG Jing,et al. Department of Gynecology,The Second Affiliated Hospital,Sun Yat-Sen University,Guangzhou 510120,China |
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| Abstract: | Objective To observe the inhibitive effect of anti-oncoprotein monoclonal antibody labeled with 131I on ovarian carcinoma cell line, SKOV3. Methods Anti-c-erbB-2 monoclonal antibody (McAb) was labeled with 131I using Chloramine-T. SKOV3 cells were cultured with 131I-anti-c-erbB-2-McAb, and the inhibition was detected with flow cytometry. Results 131I-anti-c-erbB-2-McAb could inhibit the growth of SKOV3 cells, the effect of 131I-normal murine IgG (nmIgG, as control agent) was rather poor, while anti-c-erbB-2-McAb without 131I could hardly inhibit the growth of SKOV3 cells in vitro. Conclusion 131I-anti-c-erbB-2-McAb can obviously inhibit the growth of the tumor cell line and is promising to be used in the targeting radioimmunotherapy. |
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| Keywords: | Ovarian neoplasms Tumor cells cultured Antibodies monoclonal Radioimmunotherapy Iodine radioisotopes |
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