纳洛酮对丙泊酚致小鼠学习记忆障碍、催眠作用的影响

目的：探讨纳洛酮对丙泊酚致小鼠学习记忆障碍以及催眠作用的影响。方法：小鼠腹腔注射不同剂量的丙泊酚（10,100 mg·kg^-1）建立模型,跳台、避暗实验中观察不同剂量的纳洛酮sc对小鼠跳台潜伏期（stepdown latency,SDL）、步入潜伏期（step through latency,STL）和错误次数的影响;催醒实验中观察不同剂量的纳洛酮皮下注射（subcutaneously,sc）对小鼠睡眠潜伏期和持续时间（sleeping time,ST）的影响。结果：跳台、避暗实验中,用药后第1 d,纳洛酮0.5 mg·kg^-1sc能够延长丙泊酚所致记忆障碍小鼠的SDL、STL,减少错误次数（P〈0.05;P〈0.01）,用药后第2、3、4 d,各组SDL、STL和错误次数的差异均无显著性（P〉0.05）;催醒实验中,纳洛酮0.5 mg·kg^-1sc对丙泊酚催眠小鼠的潜伏期和ST没有明显影响（P〉0.05）,纳洛酮1.2 mg·kg^-1sc能够延长丙泊酚催眠小鼠的潜伏期,缩短其ST（P〈0.05;P〈0.01）。结论：纳洛酮可以改善丙泊酚所致的学习记忆障碍,可以减轻丙泊酚的催眠作用。

Effect of Naloxone on Propofol-induce Learning Deficit and Dysmnesia and Hypnosis of Mice

Objective：To investigate the effect of naloxone on propofol-induced learning deficit and dysmnesia and hypnosis of mice. Methods：Animal models were established by intraperitoneal injecting（ip） of propofol（10 and 100 mg·kg^-1）.Normal saline or different doses of naloxone were subcutaneously injected（sc）,then the step down latency（SDL）,error times in step down test,step through latency（STL）,error times in step through test were observed and recorded;and sleeping latency and time（ST） in analeptic test were recorded. Results：In step down test and step through test,the first day after treatment,naloxone 0.5 mg·kg^-1（sc） increased the SDL and STL,and decreased the error times of mice treated with propofol（P0.05;P0.01）;2,3,4 days after drug,there was no significant difference in SDL,STL,and error times of each group（P0.05）;In analeptic test,naloxone 0.5 mg·kg^-1（sc） on propofol hypnosis in mice did not affect the latency and ST（P 0.05）,naloxone 1,2 mg·kg^-1（sc） extend the sleeping latency and decreased the ST（P0.05;P0.01）. Conclusions：naloxone could improve propofol-induced learning deficit and dysmnesia and abate the hypnosis of propofol.