On the possibility of photodynamic inactivation of tracheobronchial tree pathogenic microbiota using methylene blue (in vitro study) |
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| Affiliation: | 1. Gamaleya National Research Center of Epidemiology and Microbiology, Moscow, 123098 Russia;2. Prokhorov General Physics Institute of the Russian Academy of Sciences, Moscow, 119991 Russia;3. National Research Nuclear University “MEPhI”, Moscow, 115409 Russia;4. I.M. Sechenov First Moscow State Medical University, Moscow, 119992 Russia;5. Federal Research Center «All-Russian Research Institute of Experimental Veterinary Medicine n.a. K.I. Skryabin and Y.R. Kovalenko» of the Russian Academy of Sciences, Moscow, 109428 Russia;1. Department of Ophthalmology, Hospital Clínico San Carlos. Instituto de Investigación Sanitaria (IdISSC), Madrid, Spain;2. Department of Ophthalmology, Centro Internacional de Oftalmología Avanzada, Madrid, Spain;3. Department of Computational, Centro de Investigacion CITIC, Universidade da Coruña, A Coruña, Spain;4. Department of Computational, VARPA Research Group, Instituto de Investigación Biomédica de A Coruña (INIBIC), Universidade da Coruña, A Coruña, Spain;1. Eye Unit, Department of Medicine, Surgery and Dentistry, \"Scuola Medica Salernitana\", University of Salerno, Baronissi, Italy |
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| Abstract: | BackgroundThe treatment of patients after mechanical ventilation of lungs suffering from a multi-species infection of the tracheobronchial tree can be complicated.. The situation is aggravated in patients with post-intubation tracheal stenosis, where infection plays a leading pathogenetic role in damage to the tracheal wall. As a result of such a pathological process, cicatricial stenosis of the trachea of purulent-inflammatory infectious genesis or infected tracheal stenosis (ITS) may occur.MethodsIn this work, we studied the possibility of photodynamic inactivation of pathogenic microbiota typical for patients with ITS using methylene blue (MB) as a photosensitizer.Results13 clinical isolates of 8 species of bacteria from 9 patients were susceptible to photodynamic inactivation with MB. 30 μM of MB at a light irradiation dose of 25 J/cm2 and incubation with MB for 15 min allows to completely inactivate bacteria found in the tracheobronchial secretions of patients with ITS.ConclusionsMB retains its optico-physical properties in the range of 3–30 μM and provides effective inactivation of isolated Gram-positive and Gram-negative bacteria, including multi- and pan-resistant to antibiotics. |
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| Keywords: | Antimicrobial Photodynamic Therapy |
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