| 白英提取物诱导人肝癌BEL—7404细胞凋亡作用 |
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| 引用本文： | 单长民,胡娟娟,杜冠华.白英提取物诱导人肝癌BEL—7404细胞凋亡作用[J].中国临床药理学与治疗学,2001,6(3):200-203. |
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| 作者姓名： | 单长民 胡娟娟 杜冠华 |
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| 作者单位： | 1. 山东滨州医学院生物学教研室
2. 中国医学科学院药物研究所 |
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| 基金项目： | 国家自然科学基金资助项目(№3001161940),科技部科技攻关项目(№96-901-02-09). |
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| 摘 要： | 探讨中药白英提取物对肝癌BEL－7404细胞系的凋亡作用。方法 用不同浓度的白英提取物处理肝癌BEL－7404细胞，通过观察细胞形态、DNA凝胶电泳法研究白英提取物诱导肝癌BEL－7404细胞产生凋亡现象。结果 光学显微镜下可见细胞呈凋亡特征性变化，凝胶电泳可见细胞DNA有规律断裂形成梯形图谱。结论 白英可诱导肝癌BEL－7404细胞产生凋亡。
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| 关 键 词： | 白英提取物 肝癌细胞BEL－7404 细胞凋亡 肿瘤治疗 |
| 修稿时间： | 2001年5月14日 |
Apoptosis of hepotoma cell line BEL-7404 induced by extracts of solanum lyratum thumb |
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| SHAN Chang Min HU Juan Juan,DU Guan Hua.Apoptosis of hepotoma cell line BEL-7404 induced by extracts of solanum lyratum thumb[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2001,6(3):200-203. |
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| Authors: | SHAN Chang Min HU Juan Juan DU Guan Hua |
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| Affiliation: | SHAN Chang Min HU Juan Juan 2 DU Guan Hua 2 |
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| Abstract: | Aim To study the biological effect of extracts of solanum lyratum thumb on inducing apoptosis of BEL 7404 cell line. Methods The BEL 7404 cells were cultured in DMED medium and treated with extract of solanum lyratum thumb and then were observed with microscope and their DNA was assayed by the method of gel. Results Treatment by extract of solanum lyratum thumb could induce the cells to reveal a typical apoptotic appearance. Their DNA was fracted and a ladder pattern characteristic could be found by the electrophoresis. The extract of solanum lyratum thumb show the same effects as paclitaxol. Conclusion solanum lyratum thumb can induce apoptosis in BEL 7404 cells. |
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| Keywords: | solanum lyratum thumb human hepotoma cell line BEL 7404 apoptosis |
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