| 赛庚啶对大鼠脑缺血再灌注损伤的保护作用及对神经细胞凋亡的影响 |
| |
| 引用本文： | 谭婷,王美纳,马小亚.赛庚啶对大鼠脑缺血再灌注损伤的保护作用及对神经细胞凋亡的影响[J].中国临床药理学与治疗学,2011,16(10):1096-1100. |
| |
| 作者姓名： | 谭婷 王美纳 马小亚 |
| |
| 作者单位： | 1. 西安交通大学医学院第二附属医院科研部,西安710004,陕西
2. 西安交通大学医学院第二附属医院药剂科,西安710004,陕西 |
| |
| 摘 要： | 目的:探讨赛庚啶(Cyproheptadine,CYP)对大鼠局灶性脑缺血再灌注损伤的保护作用及机制。方法:利用Longa线栓法制作大鼠局灶性脑缺血3h再灌注3、6、24、48h模型,TTC染色观察脑梗死体积,图像分析系统测定脑梗死范围,紫外分光光度仪测定血清乳酸脱氢酶(LDH)含量,TUNEL法检测凋亡神经细胞,免疫组化法测定脑细胞Caspase-3的表达。结果:与模型组比较,CYPI、II组的脑梗死绝对容积及梗死百分比显著减小(P<0.01),由于缺血再灌注导致的血清LDH含量升高显著降低(P<0.01),凋亡神经细胞及Caspase-3阳性表达产物明显减少(P<0.01)。结论:CYP对大鼠脑缺血再灌注损伤具有保护作用,抑制神经细胞凋亡过程,其机制与CYP抑制血清LDH升高及减少Caspase-3表达有关。
|
| 关 键 词： | 赛庚啶 脑缺血再灌注损伤 保护作用 神经细胞凋亡 Caspase-3 |
Effects of Cyproheptadine on cerebral ischemia-reperfusion injury and nerve cell apoptosis |
| |
| TAN Ting,WANG Mei-na,MA Xiao-ya.Effects of Cyproheptadine on cerebral ischemia-reperfusion injury and nerve cell apoptosis[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2011,16(10):1096-1100. |
| |
| Authors: | TAN Ting WANG Mei-na MA Xiao-ya |
| |
| Affiliation: | TAN Ting1,WANG Mei-na2,MA Xiao-ya1 1 Ministry of Scientific Research,2 Department of Pharmacy,Second Affiliated Hospital,Medical School of Xi'an Jiaotong University,Xi'an 710004,Shaanxi,China |
| |
| Abstract: | AIM:To study the protection and mechanism of Cyproheptadine(CYP) on focal cerebral ischemia-reperfusion in rats. METHODS:The models with cerebral ischemicre-reperfusion were induced by intraluminal middle cerebral artery occlusion(MCAO) with a nylon monofilament suture,2 h occlusion followed by 3,6,24,48 h reperfusion.The volume of cerbral infarction was observed using 2,3,4-triphenyltetrazolium chloride(TTC) dyeing,apoptosis of the neurocytes was detected by TUNEL technique,the expression levels of Caspase... |
| |
| Keywords: | Cyproheptadine Cerebral ischemia-reperfusion injury Protect effect Nerve cell apoptosis Caspase-3 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
