药物联合治疗对肾血管性高血压大鼠左室重构影响的对比研究

目的：通过两两药物联合应用治疗高血压,观察并对比药物联合治疗的降压效果,并探讨其对左室重构的作用。方法：用两肾一夹法建立高血压大鼠模型,43只SD大鼠随机分为：假手术组（A组,n=10）,高血压组（B组,n=11）,β-B＋ACEI组（C组,n=11）予以比索洛尔5mg·kg-1.d-1及贝那普利5mg·kg-1·d-1灌胃,ACEI＋CCB组（D组,n=11）予以贝那普利5mg·kg-1·d-1及氨氯地平2.5mg·kg-1·d-1灌胃,饲养20周,期间每两周给大鼠测量鼠尾动脉压和超声心动图,20周后采集标本,用免疫组化法检测胶原Ⅲ、胶原Ⅳ及心钠素（ANF）。结果：用药组血压、胶原Ⅲ、胶原Ⅳ含量较B组明显降低,差异具有统计学意义。与A组比较,B组ANF明显升高（P〈0.05）,各药物治疗组ANF无明显升高;与B组比较,各药物治疗组ANF显著降低（P〈0.01）。结论：在降压和靶器官保护方面,贝那普利和氨氯地平联用疗效优于贝那普利和比索洛尔联用。

Comparative study of combined medication on improving left ventricular remodeling in renal vascular hypertension rats

AIM:To investigate the effect of combined medication on treating hypertension and explore its role in left ventricular remodeling in renal vascular hypertension rats(two kidney one clip hypertensive rats ).METHODS:Forty-three SD rats were randomly divided into 4 groups:sham group(Group A,n=10),hypertension group(Group B,n=11),β-B + ACEI group(Group C,n=11 bisoprolol 5 mg·kg~(-1)·d~(-1) and benazepril 5 mg·kg~(-1)·d~(-1)),ACEI + CCB group (Group D,n=11 benazepril 5 mg·kg~(-1)·d~(-1) and amlodipine 10 mg·kg~(-1)·d~(-1)).The rats were fed for 20 weeks, blood pressure and echocardiography of rats were monitored every 2 weeks.After 20 weeks,(rats were sacrificed and the blood and hearts were collected,then the concentrations of collagen Ⅲ,collagen Ⅳ and ANF were detected by immunohistochemistry.RESULTS:Compared with Group B,the blood pressure,the concentrations of collagen Ⅲ,collagen Ⅳ and ANF in medication groups were significantly reduced.Compared with Group A,the ANF was significantly increased in Group B,but the ANF was not significantly increased in medication groups(P＜0.05).CONCLUSION:The combined effect of benazepril and amlodipine is better than bisoprolol and benazepril in reducing blood pressure and preventing target organ.