| 降钙素基因相关肽对大鼠血管平滑肌细胞CDK2和Cyclin E的影响 |
| |
| 引用本文： | 邓水秀,曾泗宇,任俊芳,郑元斌,廖端芳,秦旭平.降钙素基因相关肽对大鼠血管平滑肌细胞CDK2和Cyclin E的影响[J].中国临床药理学与治疗学,2011,16(3):249-253. |
| |
| 作者姓名： | 邓水秀 曾泗宇 任俊芳 郑元斌 廖端芳 秦旭平 |
| |
| 作者单位： | 1. 湖南环境生物职业技术学院药学系,衡阳,421005,湖南
2. 南华大学药物药理研究所,衡阳,421001,湖南 |
| |
| 基金项目： | 国家自然科学基金，湖南省自然科学基金 |
| |
| 摘 要： | 目的:研究降钙素基因相关肽(CGRP)对大鼠血管平滑肌细胞中细胞周期蛋白依赖性激酶2(cyclin-dependent kinase 2,CDK2)和细胞周期蛋白E(Cyclin E)的影响,为阐明CGRP抑制血管平滑肌细胞增殖的细胞周期机制提供实验依据。方法:培养大鼠胸主动脉血管平滑肌细胞,分别用CGRP或/和10%FBS处理细胞。噻唑蓝比色法(MTT)观察CGRP对10%FBS诱导大鼠血管平滑肌细胞增殖的影响;流式细胞术分析细胞周期;Western blot检测CDK2和Cyclin E的表达。结果:CGRP能抑制10%FBS诱导的血管平滑肌细胞增殖,升高G0/G1期细胞百分比,降低S+G2+M期细胞百分比,抑制细胞内CDK2和Cyclin E表达。结论:CGRP能通过阻滞细胞周期由G0/G1期进入S期而抑制血管平滑肌细胞增殖,其作用机制可能与降低CDK2和Cyclin E表达有关。
|
| 关 键 词： | 降钙素基因相关肽 血管平滑肌细胞 细胞周期 细胞周期蛋依赖性激酶 细胞周期蛋白E |
Effect of CGRP on the CDK2 and Cyclin E of rat vascular smooth muscle cell |
| |
| DENG Shui-xiu,ZENG Si-yu,REN Jun-fang,ZHENG Yuan-bin,LIAO Duan-fang,QIN Xu-ping.Effect of CGRP on the CDK2 and Cyclin E of rat vascular smooth muscle cell[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2011,16(3):249-253. |
| |
| Authors: | DENG Shui-xiu ZENG Si-yu REN Jun-fang ZHENG Yuan-bin LIAO Duan-fang QIN Xu-ping |
| |
| Affiliation: | DENG Shui-xiu1,ZENG Si-yu2,REN Jun-fang2,ZHENG Yuan-bin2,LIAO Duan-fang2,QIN Xu-ping21 Department of Pharmacy,Hunan Environment-Biological Polytechnic College,Hengyang 421005,Hunan,China,2 Institute of Pharmacy and Pharmacology,University of South China,Hengyang 421001 |
| |
| Abstract: | AIM:To study the effect of calcitonin gene-related peptide(CGRP) on cyclin-dependent kinase 2(CDK2) and Cyclin E of vascular smooth muscle cell(VSMC),and to elute the mechanism of inhibitory effect of CGRP on proliferation of VSMC in cell cycles.METHODS: VSMC were prepared from thoracic aortas of male Sprague-Dawley rat by explanting method.The cell viability and cell cycle were determined by MTT and Flow Cytometry,respectively.Western Blot was used to observe expressions of CDK2 and Cyclin E of VSMC.RESULT... |
| |
| Keywords: | CGRP Vascular smooth muscle cell Cell cycle CDK2 Cyclin E |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
