| 三氧化二砷通过JNK信号通路诱导K562细胞凋亡 |
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| 引用本文： | 章圣辉,韩义香,吴建波,叶爱芳,尹丽慧,谭映霞.三氧化二砷通过JNK信号通路诱导K562细胞凋亡[J].中国临床药理学与治疗学,2008,13(10). |
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| 作者姓名： | 章圣辉 韩义香 吴建波 叶爱芳 尹丽慧 谭映霞 |
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| 作者单位： | 温州医学院附属第一医院医学科学研究所,温州,325000,浙江 |
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| 摘 要： | 目的:探讨c-Jun氨基末端激酶(JNK)信号通路在三氧化二砷(As2O3)诱导K562细胞凋亡中的作用及机制。方法:体外培养K562细胞,用As2O3及特异性JNK抑制剂SP600125对K562细胞进行处理;倒置相差显微镜下观察细胞形态学变化;MTT法检测不同时间点细胞增殖抑制率;AnnexinV/PI染色结合流式细胞术检测细胞凋亡率;ELISA检测p-JNK蛋白表达的变化;流式细胞术检测突变型P53表达。结果:ELISA显示4μmol/LAs2O3作用48h后p-JNK蛋白表达增强,经SP600125预处理后,As2O3诱导的K562细胞p-JNK蛋白表达明显减弱(P<0.01),As2O3诱导的细胞增殖抑制率和细胞凋亡率均下降,与As2O3单作用组相比突变型P53表达增加(P<0.05)。结论:JNK信号转导通路在As2O3诱导K562细胞凋亡过程中发挥重要作用,是As2O3诱导K562细胞凋亡的主要途径之一。
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| 关 键 词： | 三氧化二砷 人慢粒细胞系 K562 细胞凋亡 p-JNK |
Apoptosis in K562 cells induced by arsenic trioxide via JNK signaling pathway |
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| ZHANG Sheng-hui,HAN Yi-xiang,WU Jian-bo,YE Ai-fang,YIN Li-hui,TAN Ying-xia.Apoptosis in K562 cells induced by arsenic trioxide via JNK signaling pathway[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2008,13(10). |
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| Authors: | ZHANG Sheng-hui HAN Yi-xiang WU Jian-bo YE Ai-fang YIN Li-hui TAN Ying-xia |
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| Affiliation: | ZHANG Sheng-hui; HAN Yi-xiang; WU Jian-bo; YE Ai-fang; YIN Li-hui; TAN Ying-xia Institute of Medical Science; the First Affiliated Hospital of Wenzhou Medical College; Wenzhou 325000; Zhejiang; China; |
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| Abstract: | AIM:To investigate the role of c-jun N-terminal kinase(JNK) signaling pathway in the apoptosis of human chronic myelogenous leukemia cell line K562 induced by arsenic trioxide and the possible mechanisms.METHODS:K562 cells were pre-incubated with SP600125 for 3 h prior to exposure to arsenic trioxide at 4 μmol/L and for different time.The cell morphological changes were observed by inverted phase contrast microscopy.The cell growth inhibitory rate was detected by MTT colorimetric assay.The apoptosis rate wa... |
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| Keywords: | arsenic trioxide human chronic myelogenous leukemia cell line K562 apoptosis c-jun terminal kinase |
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