茶多酚对胶原诱导性关节炎大鼠足爪组织MMP-2蛋白表达水平的影响

目的：探讨茶多酚对Ⅱ型胶原乳化剂诱导关节炎大鼠的治疗作用及机制。方法 SD大鼠随机分为正常对照组、模型组、地塞米松对照组（2 mg·kg－1·d－1）和茶多酚治疗组（200 mg·kg－1·d－1），采用Ⅱ型胶原弗氏完全佐剂法（collagen-in-duced arthritis，CIA）建立类风湿性关节炎大鼠模型。记录大鼠足趾体积的变化，酶联免疫法（ELISA）检测大鼠血清IL-1β、PGE2水平，免疫组化法检测大鼠足爪组织基质金属蛋白酶2（MMP-2）蛋白水平的表达。结果与模型组比较，茶多酚治疗组大鼠踝关节肿胀明显减轻（P＜0．05），大鼠血清IL-Iβ、PEG2水平明显降低（P＜0．05），足爪组织细胞中MMP-2蛋白表达明显下降（P＜0．05）。结论茶多酚对CIA大鼠关节炎有抑制作用，其机制可能与抑制炎症介质IL-1β、PEG2，下调MMP-2蛋白表达有关。

Effect of Tea Polyphenols on paw MMP-2 protein expression in collagen-induced arthritis rats

Objective To investigate the effect of Tea polyphenols on collagen-induced arthritis(CIA)rats,and to explore its therapeutic mechanism.Methods SD rats were randomized into four groups:normal control group,model group,dexamethasone group (2 mg · kg-1 ·d-1 )and tea polyphenols group(200 mg·kg-1 ·d-1 ).The rats were given pedal intradermal injection of collagen and Com-plete Freund's Adjuvant to induce arthritis.We recorded the changes of rats in toe volume .The serum content of IL-1βand PGE2 were assayed by ELISA,and the expression of MMP-2 on rats paw was detected by immunohistochemical staining.Results As compared with the model group,the ankle joint swelling degree was relieved in tea polyphenols group (P<0.05 ),the serum levels of IL-1β&PEG2 were significantly decreased(P<0.05),and the expression of MMP-2 protein on paw also decreased(P<0.05).Conclusions Tea polyphenols has an obvious inhibition on CIA rats,and its mechanism may be related to the reduction of IL-1 β&PEG2 ,and down-regulation of MMP-2.