胃癌中uPA、肿瘤浸润性巨噬细胞的表达及其与血管生成的关系

目的探讨胃癌组织中尿激酶型纤溶酶原激活物（uPA）的表达和肿瘤相关巨噬细胞（TAMs）的浸润与胃癌的发生发展和血管生成的关系。方法采用免疫组化SP法检测20例胃炎组织及50例不同分化程度胃癌组织中TAMs、微血管密度（MVD）及uPA的表达。结果（1）胃腺癌中uPA的阳性表达率明显高于胃炎组织及印戒细胞癌（P〈0．01），且与胃癌的转移有关（P〈0．05）。（2）胃癌中TAMs计数显著高于胃炎组织（P〈0．01），且有淋巴结转移组TAMs计数高于无淋巴结转移组，差异有统计学意义（P〈0．05）。（3）uPA、TAMs及MVD三者存在正相关性（P〈0．05）。结论uPA、TAMs及MVD三者协同作用促进肿瘤的发生发展及转移。

Expression of uPA and TAMs in gastric cancer and their relationship with the tumor angiogenesis

Aim To investigate the expression of urokinase-type plasminogen activator （uPA） and the infiltration of tumor-associated macrophages（TAMs） in human gastric cancer and their relationship to the tumor progression and angiogenesis. Methods TAMs,mi- crovessel density（MVD） and uPA were detected by immunohistochemistry streptavidinperoxidase method（ S-P method） in 20 gastritis and 50 differentiated gastric cancer. Results First, uPA positive expression in gastric adenocarcinoma was higher than that in the gastritis and signet-ring cell carcinoma （ P 〈 0. 01 ） , and uPA positive expression was concerned with the metastasis of gastric cancer （ P 〈 0. 05 ）. Second,TAMs counts in gastric cancer were more than those in gastritis （P 〈 0. 01 ） , and in lymph node metastasis group,TAMs counts were more than those in no-metastasis group （P 〈 0. 05 ）. Third, uPA, TAMs and MVD had positive correlation with each other（ P 〈 0. 05 ）. Conclusion uPA,TAMs and MVD have synergistic effect with each other in development and metastasis of gastric cancer.