灯盏花素对高糖环境肾系膜细胞c-fos、c-jun蛋白表达的影响

目的　观察高葡萄糖环境中 ,蛋白激酶C(PKC)抑制剂灯盏花素对肾小球系膜细胞 (GMC)c fos、c jun蛋白表达和Ⅳ型胶原 (C Ⅳ )合成的影响 ,探索糖尿病肾病防治的新途径。方法　原代培养大鼠GMC ,分别置于正常葡萄糖 (对照组 )、高葡萄糖 (高糖组 )和高葡萄糖加灯盏花素 (高糖加灯盏花素组 )环境中 ,观察干预 2 4h、4 8h和 1wk后GMCc fos、c jun蛋白表达、C Ⅳ合成和PKC活性的变化。结果　与对照组比较 ,高糖组干预 2 4h后c fos、c jun蛋白表达同时明显增高 ,4 8h后c fos开始下降 ,而c jun 1wk后仍保持高水平 ,高糖组C Ⅳ合成 1wk后增加 ,各观察时点PKC活性均较对照组明显增高 ;而高糖加灯盏花素组各时点c fos、c jun蛋白表达、C Ⅳ合成和PKC活性均低于高糖组。结论　高葡萄糖可促使GMC中c fos、c jun蛋白表达和C Ⅳ合成增加 ,此可能为PKC活化所介导 ,灯盏花素可通过抑制PKC活化而有效阻止高葡萄糖引起的上述变化。

Effects of breviscapine on protein expression of c-fos,c-jun in glomerular mesangial cells cultured under high glucose conditions

AIM To explore effects of protein kinase C (PKC) inhibitor breviscapine on protein expression of c fos?c jun and collagen Ⅳ(C Ⅳ) production in rat glomerular mesangial cells (GMC) cultured under high glucose conditions. METHODS Rat GMCs were cultured under normal glucose, high glucose or high glucose and breviscapine. After 24 h, 48 h or 1 wk, protein expression of c fos?c jun, C Ⅳ and PKC activity were measured. RESULTS Increased protein expression of c fos?c jun, C Ⅳ and PKC activity were observed under high glucose compared to normal glucose. All the above mentioned effects of high glucose were inhibited completely or partly by breviscapine. CONCLUSION High glucose may increase protein expression of c fos?c jun and C Ⅳ production mediated by PKC activation in cultured GMCs. PKC inhibitor breviscapine may ameliorate these abnormalities efficiently.