| 当归A_3活性部位对小鼠巨噬细胞环氧化酶-2活性及基因表达的影响 |
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| 引用本文： | 沈建芬,张又枝,肖军花,王嘉陵.当归A_3活性部位对小鼠巨噬细胞环氧化酶-2活性及基因表达的影响[J].中国药理学通报,2011,27(11):1574-1577. |
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| 作者姓名： | 沈建芬 张又枝 肖军花 王嘉陵 |
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| 作者单位： | 1. 浙江省嘉兴市第一医院,浙江,嘉兴,314000
2. 华中科技大学同济医学院基础医学院药理学系,武汉,430030 |
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| 摘 要： | 目的研究当归A3活性部位对小鼠巨噬细胞RAW264.7环氧化酶-2(cyclooxygenase-2,COX-2)活性及基因表达的影响。方法采用酶联免疫法(enzyme-line immu-nosorbnent assay,ELISA)检测前列腺素E2(prostaglandin E2,PGE2)产量及COX-2活性,采用RT-PCR和Western blot法检测COX-2 mRNA及蛋白表达水平。结果 A3(20、40、80mg.L-1)剂量依赖性地抑制LPS诱导的RAW264.7细胞PGE2产量、COX-2活性、COX-2 mRNA及蛋白表达水平增高。结论 A3能够直接抑制PGE2产量,此作用可能与抑制COX-2基因表达有关。
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| 关 键 词： | 当归 A3活性部位 环氧化酶-2 脂多糖 前列腺素E2 巨噬细胞 |
Effect of angelica A3 active component on activity and expression of cyclooxygenase-2 in mouse RAW264.7 macrophage cells |
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| SHEN Jian-fen,ZHANG You-zhi,XIAO Jun-hua,WANG Jia-ling.Effect of angelica A3 active component on activity and expression of cyclooxygenase-2 in mouse RAW264.7 macrophage cells[J].Chinese Pharmacological Bulletin,2011,27(11):1574-1577. |
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| Authors: | SHEN Jian-fen ZHANG You-zhi XIAO Jun-hua WANG Jia-ling |
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| Affiliation: | SHEN Jian-fen1,ZHANG You-zhi2,XIAO Jun-hua2,WANG Jia-ling2(1.the First Hospital of Jiaxing,Jiaxing Zhejiang 314000,China,2.Dept of Pharmacology,Tongji Medical College of Huazhong University of Science and Technology,Wuhan 430030,China) |
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| Abstract: | Aim To investigate effects of angelica A3 active component(A3) on cyclooxygenase-2(COX-2) activity and expression in lipopolysaccharide(LPS)-induced mouse RAW264.7 macrophage cells.Methods Prostaglandin E2(PGE2) production and COX-2 enzyme activity were investigated by enzyme-line Immunosorbnent assay(ELISA).RT-PCR and Western blot were used to analyze cyclooxygenase-2 mRNA and the protein expression level.Results A3 20,40,80 mg·L-1 was shown to markedly inhibit the LPS-induced PGE2 production,COX-2 enzyme activity,COX-2 mRNA and protein expression in RAW264.7 cells(P<0.05).Conclusion A3 can directly decrease PGE2 production,which may be related to inhibiting COX-2 gene expression. |
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| Keywords: | angelica A3 active component cyclooxygenase-2 lipopolysaccharide prostaglandin E2 macrophage cells |
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