| 蛋白激酶Cδ亚型抑制剂对脂多糖诱导肺微血管内皮细胞损伤的影响 |
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| 引用本文： | 朱钟鸣,孙耕耘.蛋白激酶Cδ亚型抑制剂对脂多糖诱导肺微血管内皮细胞损伤的影响[J].中国药理学通报,2006,22(7):800-803. |
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| 作者姓名： | 朱钟鸣 孙耕耘 |
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| 作者单位： | 安徽医科大学第一附属医院呼吸内科,安徽,合肥,230022 |
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| 基金项目： | 国家高技术研究发展计划(863计划);安徽省卫生厅资助项目 |
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| 摘 要： | 目的研究蛋白激酶C(PKC)的亚型PKCδ在大鼠肺微血管内皮细胞(RPMVEC)中的表达及其抑制剂Rottlerin对脂多糖(LPS)导致RPMVEC单层通透性增高的影响。方法取体外培养的RPMVEC进行PKCδ的W estern b lot和免疫组化检测,用针头式滤器检测LPS及LPS+Rottlerin干预后RPMVEC单层滤过系数(K f)的变化。结果PKCδ在RPMVEC中有广泛的表达,其表达部位主要位于胞质。Rot-tlerin能减低LPS导致的RPMVEC单层通透性增高。结论LPS导致RPMVEC损伤的机制与PKC的激活有关,PKCδ亚型抑制剂可减轻LPS诱导的RPMVEC单层通透性增高。
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| 关 键 词： | 蛋白激酶C 脂多糖类 内皮细胞 通透性 |
| 文章编号： | 1001-1978(2006)07-0800-04 |
| 收稿时间： | 02 21 2006 12:00AM |
| 修稿时间： | 03 28 2006 12:00AM |
Effect of PKCδ inhibitor on permeability injury of pulmonary microvascular endothelial cells monolayer induced by lipopolysaccharide |
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| ZHU Zhong-ming,SUN Geng-yun.Effect of PKCδ inhibitor on permeability injury of pulmonary microvascular endothelial cells monolayer induced by lipopolysaccharide[J].Chinese Pharmacological Bulletin,2006,22(7):800-803. |
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| Authors: | ZHU Zhong-ming SUN Geng-yun |
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| Affiliation: | Dept of Respitatory Medicine,The 1 st Affiliated Hospital of Anhui Medical University ,Hefei 230022,China |
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| Abstract: | Aim To study the expression of protein kinase C(PKC) subtype PKCδ in rat pulmonary microvascular endothelial cell(RPMVEC),and the effect of its inhibitor(Rottlerin) on LPS-induced RPMVEC monolayer permeability injury.Methods In cultured RPMVEC,Western blot and immunohistochemistry were used to identify the expression of PKCδ.Micro-infiltrator was used to measure the changes of RPMVEC monolayer permeability coefficient(Kf) after exposure to LPS or LPS with Rotterin together.Result We found a high level of PKCδ expression in cytoplasm.Rottlerin could obviously inhibit the increase of LPS-induced RPMVEC Kf value.Conclusion The activation of PKC is involved in the progress of LPS-induced RPMVEC injury.Furthermore,the inhibitor of PKCδ could release LPS-induced RPMVEC Kf increase. |
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| Keywords: | protein kinase C lipopolysaccharides endothelial cells permeability |
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