四逆汤对肾性高血压大鼠血压的调节作用和机制

目的探讨四逆汤对肾性高血压大鼠血液内血管活性物质含量和主要靶器官血管活性物质表达的调节作用。方法健康♀Wistar大鼠24只,随机分为伪手术(SO)组、肾性高血压(RH)组和四逆汤治疗(ST)组,每组8只。根据双肾动脉夹闭法建立肾性高血压动物模型,应用RBF-2型大鼠尾动脉血压计测量血压,硝酸还原酶比色法测定血清中一氧化氮(NO)含量,放射免疫法测定血浆中内皮素(ET)、降钙素基因相关肽(CGRP)和血管紧张素Ⅱ(AngⅡ)的含量。免疫组化法测定心、脑、肾组织内皮素受体A(ETRA)、一氧化氮合酶(NOS)、AngⅡ和CGRP的表达。结果①ST组大鼠血压明显低于RH组(P<0.05)。②大鼠血清NO、血浆CGRP和ET含量ST组均明显高于RH组(P<0.05),AngⅡ含量无变化(P>0.05)。③大鼠心、肾ETRA表达水平ST组均明显低于RH组(P<0.05),但脑组织中含量差异无显著性(P>0.05);心脏eNOS表达水平ST组明显高于RH组(P<0.05),脑、肾iNOS表达水平ST组明显低于RH组(P<0.05);心、脑、肾CGRP表达水平ST组均低于RH组(P<0.05);心、肾AngⅡ表达水平ST组均明显低于RH组(P<0.05),但在脑差异无显著性(P>0.05)。结论四逆汤可能通过调节肾性高血压大鼠心、脑、肾内血管活性物质的表达及其血液内的有效含量,而发挥血压调节作用。

The regulating effect and mechanism of Sini decoction on vascular active substance in renovascular hypertensive rats

Aim To investigate the regulating effect and mechanism of Sini decoction(SND)on the vasoactive substances expression in main target organs and the contents in blood.Methods A total of 24 healthy female Wistar rats were randomly divided into sham operation(SO)group,renovascular hypertension(RH)group and SND treatment(ST)group consisting of 8 cases in each group.The renovascular hypertensive rat models were established according to two-kidney two-clip(2K2C)method.The blood pressure of rats was measured by RBF-2 tail blood pressure meter.The content of nitric oxide(NO)in serum was determined by nitrate reductase assay;and the contents of endothelin(ET),calcium gene related peptide(CGRP)and angiotensinⅡ(AngⅡ)in plasma were determined by radioimmunoassay.The expressions of endothelin receptor A(ETRA),nitric oxide synthesis(NOS),AngⅡ,and CGRP in heart,brain and kidney tissue were determined by immunochemistry assay.Results ① The blood pressure level in ST group was lower than that in RH group(P<0.05).② The NO contents in serum and the CGRP and ET in plasma in ST group were higher than those in RH group(P<0.05),while AngⅡ had no significant change(P>0.05).③ The expression levels of ETRA in heart and kidney in ST group were lower than those in RH group(P<0.05),but there was no significant difference in brain(P>0.05).The expression level of eNOS in heart in ST group was higher than that in RH group(P<0.05),but the expression levels of iNOS in brain and kidney in ST group were significantly lower than those in RH group(P<0.05).The expression levels of CGRP in heart,brain and kidney in ST group were markedly lower than those in RH group(P<0.05).The expression levels of AngⅡin heart and kidney in ST group were markedly lower than those in RH group(P<0.05),while there was no significant difference existed in brain(P>0.05).Conclusions SND might regulate the blood pressure in hypertensive rats by adjusting the vascular active substances expression in main target organs and the levels in blood.