梁金菇多糖促人急性早幼粒细胞白血病细胞系(HL-60)细胞凋亡研究

目的 :观察梁金菇多糖对人类早幼粒细胞白血病细胞系 (HL 6 0 )是否具有凋亡诱导作用 ,并进一步研究半胱天冬酶 3(Caspase 3)基因在此过程中的作用。方法 :四氮唑蓝比色法 (MTT法 )观察梁金菇多糖对HL 6 0细胞的抑制率 ,应用形态学、流式细胞术、DNA凝胶电泳等方法检测细胞凋亡的发生。应用半定量逆转录 聚合酶链反应 (RT PCR)法检测凋亡相关基因Caspase 3mRNA表达的变化。结果 :梁金菇多糖对HL 6 0细胞的生长有明显的抑制作用 ,并诱导肿瘤细胞发生凋亡 ;在HL 6 0细胞凋亡过程中 ,凋亡相关基因Caspase 3转录水平比用药前增强。结论 :梁金菇多糖促HL 6 0细胞凋亡 ,且与Caspase 3基因表达有关

An experimental study on Liangjin mushroom polysaccharide inducing HL-60 cell apoptosis

AIM: To observe Liangjin mushroom polysaccharide(LJPS) inducing apoptosis in human HL-60 cell and study its relationship with Caspase-3 gene. METHODS: The proliferation inhibition effects of LJPS on HL-60 cells was observed by MTT colorimetry.Apoptosis was detected by morphology, flow cytometry and DNA agarose gel electrophoresis. Caspase-3mRNA expression was detected by semi-quantitative RT-PCR. RESULTS: Liangjin mushroom polysaccharide could significantly inhibit HL-60 cell proliferation and induce its apoptosis. Caspase-3mRNA expression was upregulated during Liangjin mushroom polysaccharide inducing apoptosis. CONCLUSION: Liangjin mushroom polysaccharide could induce HL-60 cell apoptosis and be related to Caspase-3 expression.