| EAE小鼠体内γδT细胞的提取和鉴定 |
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| 引用本文： | 张金涛,尚晓玲,蒋建华,倪建强,邢广羽,刘婉莹,尹岭.EAE小鼠体内γδT细胞的提取和鉴定[J].实用医药杂志(山东),2008,25(11). |
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| 作者姓名： | 张金涛 尚晓玲 蒋建华 倪建强 邢广羽 刘婉莹 尹岭 |
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| 作者单位： | [1]88医院神经内科,山东泰安271000 [2]解放军总医院,北京100853 |
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| 基金项目： | 全军医药卫生科研基金 |
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| 摘 要： | 目的试图从蛋白脂质蛋白(Proteolipid protein,PLP)免疫后的SJL/J小鼠肠上皮间(iIEL)、淋巴结(LNC)中提取γδT细胞,并探讨γδT细胞在实验性自身免疫性脑脊髓炎(Experimental autoimmune encephalomyelitis,EAE)发病机制中的作用。方法用多肽PLP178-191免疫小鼠,从小鼠iIEL、LNC中提取γδT细胞,利用免疫荧光标记和流式细胞分析技术进行检测。结果PLP免疫组和正常对照组比较,小鼠iIEL中CD3+细胞百分比在组间无统计学差异(P>0.05),而小鼠LNC中CD3+细胞百分比在组间存在显著性差异(P<0.01);小鼠iIELCD3+细胞中和LNCCD3+细胞中γδT细胞的百分比,组间均存在显著性差异(P<0.01)。结论利用PLP178-191免疫SJL/J小鼠,可以稳定地提取γδT细胞,γδT细胞的确参与了EAE发病过程。
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| 关 键 词： | SJL/J小鼠 γδT细胞 实验性自身免疫性脑脊髓炎 |
Study on extraction and identification of γδT-cell in EAE mice |
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| Abstract: | Objective To extract γδT-cells from iIEL and LNC of SJL/J mice after immunized with PLP,and to explore its effect in EAE. Method PLP178-191 was used to immunize SJL/J mice,thenγδT-cells were extractd from iIEL and LNC. Meanwhile,immunofluorescence marker and flow cytometry analysis were performed. Result Compared PLP group with control group,there were no marked different percentages of CD3 + cell in the iIEL (P >0.05). However,there were marked different percentages of CD3+ cell in the LNC (P<0.05),and marked different percentages of γδT-cells in the CD3+ cell both in the iIEL and LNC of SJL/J mice (P<0.05). Conclusion γδT-cell can be extracted from EAE model of SJL/J mice successfully,and γδT-cells indeed are correlated with EAE. |
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| Keywords: | SJL/J mice Experimental autoimmune encephalomyelitis γδT-cell |
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