β-甘露聚糖酶产生菌DY-14的发酵优化及分离纯化 |
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引用本文: | 冯娜,赵君,赵敏.β-甘露聚糖酶产生菌DY-14的发酵优化及分离纯化[J].黑龙江医药,2011,24(3):397-401. |
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作者姓名: | 冯娜 赵君 赵敏 |
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作者单位: | 1. 东北林业大学生命科学学院,黑龙江,哈尔滨,150040 2. 哈尔滨市红十字中心血站 |
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摘 要: | 利用单因素实验和正交分析方法得到枯草芽孢杆菌DY-14发酵产酶的最佳培养基组分及条件:以魔芋粉为碳源(3%),蛋白胨为氮源(1.5%),初始pH 8.0,摇瓶装液量10 mL/100mL,接种量1%,37℃培养24h,最终产生的β-甘露聚糖酶活力可达3216 U/mL。DY-14发酵液经(NH4)2SO4盐析、DEAE-Sepharose阴离子交换层析、Sephadex G-75凝胶过滤,得到了电泳纯的β-甘露聚糖酶。
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关 键 词: | β-甘露聚糖酶 枯草芽孢杆菌 发酵优化 分离纯化 |
Optimization and purification of Beta-Mannanase by Bacillus subtilis DY-14 |
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Affiliation: | Feng Na Life Science college,Northeast Forest University(Harbin,Heilongjiang 150040,China) |
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Abstract: | Single factor and orthogonal array design experiments were performed to obtain the optimum fermentation conditions for β-mannanase production,Bacillus subtilis DY-14 grew best when cultured at 37℃with the medium composed of 3% konjac powder as source,1.5% peptone as nitrogen source,and adjusting pH to 8.0.After 24 hours culturing,the enzyme could show the highest activity as 3216 U/mL.After salting out the DY-14 ferment liquid by(NH4)2SO4,use DEAE-Sepharose for anion exchange chromatography and Sephadex G-75 for gel filter,the electrophoresis pure of β-mannanase by Bacillus subtilis DY-14 was gained. |
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Keywords: | β-mannanase Bacillus subtilis Ferment optimization Purification |
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