| 喷雾干燥对溶菌酶一级结构的影响 |
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| 引用本文： | 朱照静,邱宗荫.喷雾干燥对溶菌酶一级结构的影响[J].中国生化药物杂志,2003,24(6):279-280. |
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| 作者姓名： | 朱照静 邱宗荫 |
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| 作者单位： | 重庆医科大学,药学系,药剂学教研室,重庆,400016 |
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| 摘 要： | 目的 考察喷雾干燥对溶茵酶一级结构稳定性的影响。方法 以送料速度2.4 ml/min、进口气流温度150℃、出口气流温度70℃、雾化器转速18 000 r/min制备喷雾干燥溶茵酶。采用MALDI-TOF/MS测定喷雾干燥对溶菌酶一级结构的影响。结果 喷雾干燥溶菌酶除了具有溶菌酶相同的准分子离子峰、二聚体、三聚体、四聚体及三聚体的两电荷离子峰外,另多5个碎片离子峰。结论 喷雾干燥可造成溶菌酶一级结构破坏。
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| 关 键 词： | 溶菌酶 喷雾干燥 稳定性 |
| 文章编号： | 1005-1678(2003)06-0279-02 |
| 修稿时间： | 2002年12月16 |
Effects of spray drying on the primary structure of lysozyme |
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| ZHU Zhao-jing,QIU Zong-yin.Effects of spray drying on the primary structure of lysozyme[J].Chinese Journal of Biochemical Pharmaceutics,2003,24(6):279-280. |
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| Authors: | ZHU Zhao-jing QIU Zong-yin |
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| Abstract: | Purpose To investigate the effect of spray drying on the stability of lysozyme. Methods Lysozyme was spray dried at a flow rate of 2.4 ml/min, inlet temperature of 150℃,outlet temperature of 70℃, an atomization speed of 18 000 r/min. The effect of spray drying on the primary structure of lysozyme was analyzed by MALDI-TOF/MS. Results The spray dried lysozyme had five more fragment ion peaks than lysozyme did shown by MALDI-TOF/MS. Conclusion The primary structure of lysozyme can be degraded by spray drying. |
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| Keywords: | lysozyme spray drying stability |
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