| 蜂毒体外抗凝血活性稳定性考察 |
| |
| 引用本文： | 刘星,陈大为,张荣庆,谢丽萍,张汝华.蜂毒体外抗凝血活性稳定性考察[J].中国生化药物杂志,2004,25(1):16-19. |
| |
| 作者姓名： | 刘星 陈大为 张荣庆 谢丽萍 张汝华 |
| |
| 作者单位： | 1. 沈阳药科大学,药学院,辽宁,沈阳,110016
2. 清华大学,生物系,北京,100084 |
| |
| 摘 要： | 目的考察蜂毒体外抗凝血活性的稳定性。方法采用凝血酶原活化时间法进行测定。结果供试品在 0～ 6 .0mg/ml浓度范围内 ,凝血时间具有明显的浓度依赖关系 ,相关系数为 0 .972 2 ;蜂毒的抗凝血活性在光照及酸性条件下相对稳定 ,其水溶液在强碱性条件下易降解 ,6 0℃条件下 6h内活性相对稳定 ,在胃蛋白酶及胰蛋白酶作用下 5min开始失活。结论蜂毒在低温及生理pH条件下较稳定 ,水溶液易失活 ,在有蛋白酶存在下极易失活
|
| 关 键 词： | 蜂毒 凝血酶原活化时间 稳定性 |
| 文章编号： | 1005-1678(2004)01-0016-04 |
| 修稿时间： | 2002年12月4日 |
Studies on the stability of anti-prothrombin activity in bee venom in vitro |
| |
| LIU Xing ,CHEN Da-wei ,ZHANG Rong-qing ,XIE Li-ping ,ZHANG Ru-hua.Studies on the stability of anti-prothrombin activity in bee venom in vitro[J].Chinese Journal of Biochemical Pharmaceutics,2004,25(1):16-19. |
| |
| Authors: | LIU Xing CHEN Da-wei ZHANG Rong-qing XIE Li-ping ZHANG Ru-hua |
| |
| Affiliation: | LIU Xing 1,CHEN Da-wei 1,ZHANG Rong-qing 2,XIE Li-ping 2,ZHANG Ru-hua 1 |
| |
| Abstract: | PurposeTo observe the stability in anti-prothrombin activity in bee venom.MethodsProthrombin time test was performed to determine anti-prothrombin activity in bee venom.ResultsAt the concentration range of 0 to 6.0 mg/ml, bee venom could prolong the coagulation time in a dose-dependent manner with a coefficient of 0.9722. Anti-prothrombin activity in bee venom was stable in the acid solution or under the light, and it was not degraded at 60℃ until 6 h, but was degraded in the strong basic solution.ConclusionAnti-prothrombin activity in bee venom was stable under the physiological condition, at low temperature and under light but it was sensitive to protease. |
| |
| Keywords: | bee venom prothrombin time test stability |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
