MMP-9和CD105在胰腺癌中的表达及意义

目的探讨MMP-9，CD105在胰腺癌发生、发展及转移过程中的意义。方法采用免疫组织化学S-P法，检测MMP-9，CD105在53例胰腺癌组织（实验组）及10例正常胰腺组织（对照组）中的表达，计数微血管密度（MVD），并结合临床病理学资料进行分析比较。结果胰腺癌组织中MMP-9的阳性表达明显高于正常胰腺组织（P〈0．05）。MMP-9的表达与胰腺癌的临床分期及淋巴结有无转移有关（P〈0．05）。胰腺癌组织的微血管密度（MVD）明显高于非肿瘤性胰腺组织。CD105的表达水平与胰腺癌临床分期及病理分级有关。在有淋巴结转移的癌组织中，其MVD值高于无淋巴结转移组织（P〈0．05）。胰腺癌组织中MMP-9的表达与MVD呈正相关（P〈0．05）。结论MMP-9的表达与胰腺癌的临床分期和淋巴结转移有关。MVD值有可能作为判断胰腺癌生物学行为的潜在指标。

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Objective To explore the significance of MMP-9 and CD105 in oncogenesis, progression and metastasis of pancreatic carcinoma. Methods Immunohistochemistry methods （S-P） were applied to detect the differential expression of MMP-9, CD105 in Paraffin-embedded tumor specimens of 53 cases pancreatic carcinoma tissue and 10 cases normal pancreatic tissue. The correlations between the expression of MMP-9 and CD105, microvessel density （MVD）, and clinicop-athologic features were statistically analyzed with SPSS 12.0. Results The positive expression of MMP-9 was significantly higher in tissues of pancreatic carcinoma than in normal pancreatic tissues （P〈0.05）. The expression of MMP-9 was correlated with lymph node metastasis and clinical stages （P〈0.05）. The results of the counted number of MVD in cancer tissues revealed that the MVD of cases with pancreatic carcinoma was significantly higher than that without pancreatic carcinoma （P〈0.05）. The expression level of CD105 was positively correlated with clinical stages and histological grade （P〈0.05）. Its MVD was significantly higher in carcinoma tissues with lymph node metastasis than in that without lymph node metastasis （P〈 0.05）. There was significantly statistic correlation between the expression of MMP-9 and MVD in pancreatic carcinoma （P〈 0.05）. Conclusions The expression of MMP-9 was significantly correlated with lymph node metastasis and tumor clinical stage. MVD had significant correlation with grade of the disease, indicating its potential value of becoming a new marker to the prognosis evaluation of pancreatic carcinoma.