甘草类黄酮对脂质过氧化和活性氧自由基的作用

本文用比色法测定过氧化脂质研究甘草类黄酮(FG)的抗脂质过氧化作用,并用化学发光法和自旋捕集技术检测该药对O₂^-和OH^·的清除作用。体外实验表明:FG 2.8～25μg/ml可明显抑制小鼠肝匀浆在振荡温育条件下引起的丙二醛升高;FG 0.265～26.5μg/ml或2.58～258 μg/ml分别对碱性二甲基亚砜或黄嘌呤/黄嘌呤氧化酶体系生成的O₂^-有显著清除作用;以上作用呈浓度依赖性变化。FG 144 μg/ml或258 μg/ml分别对PMA刺激多形核白细胞释放的O₂^-及OH^·或Fenton's反应生成的OH^·有明显清除作用。实验证明FG有抗氧化作用。

EFFECTS OF GLYCYRRHIZA FLAVONOID ON LIPID PEROXIDATION AND ACTIVE OXYGEN RADICALS

The antiperoxidant activity of glycyrrhiza flavonoid (FG) was studied by using colorimetric estimation of lipid peroxide (MDA) formation. The scavenging effects of FG on O2-. and OH. was investigated by using chemiluminescence method and spin trapping technique in different systems. The results were as follows: FG 2.8-25 micrograms/ml effectively inhibited MDA formation induced by incubating mice liver homogenate at 37 degrees C for 1 h; FG 0.265-26.5 micrograms/ml or 2.58-258 micrograms/ml was shown to markedly scavenge O2-. in alkaline/DMSO or xanthine/xanthine oxidase systems, respectively in a concentration-dependent manner. FG 144 micrograms/ml or 258 micrograms/ml also significantly scavenged the active oxygen radicals produced by PMN stimulation with PMA or OH. produced in Fenton's reaction respectively. The results suggest that FG may be used as an antioxidant.