盘花垂头菊中两种新型倍半萜类化合物抑制人肝癌SMMC-7721细胞生长

1β ,8 二当归酰氧基 2 β 己酰氧基 3β ,10 二羟基 4α 氯 11 甲氧基 没药 7(14) 烯 (化合物 1)和 1β ,8 二当归酰氧基 2 β 己酰氧基 3β 羟基 4α 氯 10 ,11 二氧代 异亚丙基 没药 7(14) 烯 (化合物 2 )是从盘花垂头菊 (ＣｒｅｍａｎｔｈｏｄｉｕｍｄｉｓｃｏｉｄｅｕｍＭａｘｉｍ )中提取出来的1] 高含氧的甜没药烯型倍半萜 (ｈｉｇｈｌｙｏｘｙｇｅｎａｔｅｄｂｉｓａｂｏｌａｎｃｅｓｅｓｑｕｉｔｅｒｐｅｎｅｓ ,ＨＯＢＳ)化合物 ,其结构式分别为 :　　盘花垂头菊是多年生草本植物 ,在我国主要分布于青…

INHIBITION OF NOVEL HIGHLY OXYGENATED BISABOLANCE SESQUITERPENE COMPOUNDS FROM CREMANTHODIUM DISCOIDEUM ON THE GROWTH OF HUMAN HEPATOMA CELL LINE SMMC-7721

AIMTo study the anticancer effects of two novel highly oxygenated bisabolance sesquiterpene compounds from Cremanthodium discoideum Maxim on the human hepatoma cell line SMMC-7721. METHODSin vitro, the cytotoxity of compound 1, 2 was tested by MTT colorimetric assay, and the single-cell gel electrophoresis (SCGE) assay was used for assessing the DNA damage of SMMC-7721 cells induced by compound 1, 2. The apoptotic rate of SMMC-7721 cells was assayed by flow cytometry. RESULTSThe IC₅₀ of SMMC-7721 cells exposed to the two compounds for 72 h were (37±7) μg·mL^-1 and (22±9) μg·mL^-1, respectively. The result of SCGE showed that the two compounds (2, 4 and 8 μg·mL^-1, 48 h) were able to induce DNA damage in SMMC-7721 cells and showed dose-dependent effect. Flow cytometric analyses showed that after exposure to the two compounds for 48 h induced the apoptosis in SMMC-7721 cells. CONCLUSIONThe two novel highly oxygenated bisabolance sesquiterpene compounds showed significant inhibition effect on the growth of SMMC-7721 cells and its mechanism was probably related with the DNA damage and apoptosis induced by them.