无毒棉花籽中黄酮苷的分离与结构鉴定

目的　研究无毒棉花籽(glandlesscottonseed)中的黄酮类化学成分。方法　利用大孔树脂、聚酰胺-6、硅胶和SephadexLH-20柱色谱进行分离纯化。用UV ,MS ,¹HNMR ,¹³CNMR ,¹H-¹HCOSY ,¹H-¹³CCOSY ,HMBC和TOCSY等光谱确定化合物结构。结果　从无毒棉花籽中分离得到5个黄酮苷,经光谱鉴定化合物结构为:山奈酚-3-O-β-D-芹菜糖( 1→2 )-α-L-鼠李糖(1→6)]-β-D-葡糖苷(I)、槲皮素-3-O-β-D-芹菜糖 ( 1→2)-α-L-鼠李糖( 1→6)]-β-D-葡糖苷(II)、槲皮素-3-O-β-D-芹菜糖 ( 1→2 )-β-D-葡糖苷(III)、芦丁(IV)和陆地棉苷(V)。结论　I是一个新化合物,II和III为首次从该属植物中分离得到。

ISOLATION AND STRUCTURE IDENTIFICATION OF FLAVONOL GLYCOSIDES FROM GLANDLESS COTTON SEEDS

AIM To isolate and determine the structure of flavonol glycoside from glandless cotton seeds. METHODS The flavonol glycosides were extracted from glandless cotton seeds with 60% ethanol and purified by polyaromatic resin, polyamide, silica gel and Sephadex LH-20 on column chromatography. UV,¹H and ¹³CNMR ,¹³C-¹H COSY, HMBC, ¹H-¹H COSY and TOCSY spectra were used for the structural identification. RESULTS Five flavonol glycosides (I-V) were obtained. They were identified as kaempferol 3-O-β-D-apiosyl(1→2)-α-L-rhamnosyl(1→6)]-β-D-glucoside (I), quercetin-3-O-β-D-apiosyl(1→2)-α-L-rhamnosyl(1→6)]-β-D-glucoside (II), quercetin-3-O-β-D-apiosyl(1→2)-α-D-glucoside (III), rutin (IV) and quercetin-3-O-β-D-glucoside (V). CONCLUSION Compound I is a new natural product. Compounds II and III were isolated from this genus for the first time.