22种氨基酸的纸层析溶剂系统最佳组合选取方法的研究

本文利用“逐步组合法”在TRS-80微型电子计算机上对22种氨基酸的纸层析溶剂系统的最佳组合作出了成功的选取,共选出三组最佳溶剂系统组合,它们的系统号码分别为:7,4,2,1;7,9,4,2;21,17,4,2,其中每一组均能在任意两供试药物的Rf×100的差值大于或等于10的情况下达到该22种氨基酸的完全分离。本文也用“数值分类—信息量选取法”选取系统最佳组合,但未能获得成功的结果。

INVESTIGATION OF THE SELECTIVE METHOD FOR OPTIMAL COMBINATION OF PAPER CHROMATOGRAPHIC SOLVENT SYSTEMS OF 22 KINDS OF AMINO ACIDS

The optimal combination of paper chromatographic solvent systems of 22 kinds of amino acids was made successfully with the stepwise combination method on TRS-80 microcomputer. Three groups of optimal combination of solvent systems were selected. The numbers of optimal combination system are 7, 4, 2, 1; 17, 9, 4, 2; 21, 17, 4, 2. The 22 kinds of amino acids tested could be separated at a level that the difference of Rf × 100 values between any two samples is equal to 10 or more using each combination of the groups mentioned above. At the same time, numerical taxonomic technique-information content method was also used to select the optimal combination of the systems, but no satisfactory results were obtained.