代谢产物鉴定及汉黄芩素与汉黄芩素-7-O-葡萄糖醛酸苷的同时测定:小鼠肝脏的分布研究(英文)

汉黄芩素具有体内外抗肿瘤活性及良好的安全性。本文采用高效液相色谱串联质谱法研究了小鼠静脉注射汉黄芩素后肝脏中的代谢产物,鉴定了五个主要的代谢产物。建立了小鼠肝脏中汉黄芩素及其代谢产物汉黄芩素-7-O-葡萄糖醛酸苷的同时测定方法。肝匀浆经醋酸乙酯提取,在C₁₈色谱柱上,采用甲醇-10 mm醋酸铵（80:20,v/v）为流动相,质谱检测采用负离子选择反应监测模式。肝组织中汉黄芩素与汉黄芩素-7-O-葡萄糖醛酸苷在0.2-40μg/g范围内呈良好线性关系,方法学评价表明本法适用于动物静脉注射汉黄芩素后体内的组织分布研究。

Metabolite identification and the development of a simultaneous quantification method for wogonin and wogonin-7-O-glucuronide:application to a distribution study in mice liver

Wogonin possesses potent inhibitory activities against cancer cell growth in vitro and in vivo and has attractive safety profiles.A highly sensitive liquid chromatography coupled with tandem mass spectrometry method was developed for the identification of major metabolites in mice liver after intravenous administration of wogonin.Five metabolites were identified and biotransformation pathways were elucidated as well.Furthermore,a method was developed and validated for the simultaneous quantitatively determi...