鳖甲活性多肽的合成及对肝星状细胞增殖的抑制作用(英文)

探讨鳖甲活性多肽的固相合成及对肝星状细胞(HSC-T6)增殖的抑制作用。根据鳖甲活性多肽的序列结构,采用固相方法对多肽进行全合成,并用MALDI-TOFMS质谱对合成多肽进行分析鉴定;肝星状细胞(HSC-T6)培养至对数生长期后,加入不同浓度合成多肽作用肝星状细胞株HSC-T6,采用MTS法分析HSC-T6生长的抑制作用;使用AnnexinV-FITC/PI法检测HSC凋亡率。结果显示通过固相合成可以得到与原序列结构一致的鳖甲合成多肽;鳖甲合成多肽浓度依赖性地抑制肝星状细胞增殖,并能显著提高肝星状细胞早期凋亡率。因此,可以成功地合成鳖甲活性多肽,且对肝星状细胞增殖有明显的抑制作用。

Synthesis of an active peptide from Carapax Trionycis and its inhibitory effect on the proliferation of hepatic stellate cells

This study was aimed to synthesize an active peptide from Carapax Trionycis and to investigate its effect on the proliferation of hepatic stellate cells(HSCs).An active peptide from Carapax Trionycis,which was shown to have significant anti-hepatic fibrosis activity,was synthesized by solid phase method and characterized by MALDI-TOF MS analysis.The HSCs in log growth phase was treated with the synthetic peptide at different concentrations.Viability and apoptosis of hepatic stellate cells were determined by MTS assay and Annexin V-FITC/PI staining,respectively.The active peptide showed strong inhibition of proliferation and induction of apoptosis of HSC-T6 in a concentration-dependent manner.The results suggest that the active peptide from Carapax Trionycis could be synthesized efficiently and has significant inhibitory effect on the proliferation of HSC-T6.