基于5S-rRNA 基因间区碱基序列鉴定繁缕

目的建立快速准确鉴定民间药繁缕(Stellariamedia)的方法。方法首次利用DNA分子鉴定技术对繁缕及混淆品牛繁缕(Myosotonaquaticum)进行了5SrRNA基因间区的PCR扩增和测序。结果DNA序列和限制性酶切谱可以用于鉴定繁缕及牛繁缕。同时对石生繁缕(S.vestita)、长叶繁缕(S.longifolia)及垂梗繁缕(S.radians)进行了5SrRNA基因间区的PCR扩增和测序,认为上述5种植物各自的DNA序列和限制性酶切谱可用于繁缕属分子系统学研究。结论该方法可用于药用植物繁缕的鉴定及繁缕属分子系统学研究。

Identification of Stellaria media by PCR

Aim To provide a rapid and reliable method for identifying the fork medicine Stellaria media (Linn.) Cyr. (Herba Stellariae mediae) (Caryophyllaceae) from its adulterant Myosoton aquati-cum (L.) Fries (Herba Myosoti aquatici) (Caryophyllaceae) by polymerase chain reaction (PCR) technology. Methods A molecular genetic approach has been developed to identify S.media for the first time. 5S-rRNA spacer domain was amplified by PCR from the isolated genomic DNA, and the PCR pro-ducts were then sequenced. Results The nucleotide sequences of S.media and M.aquaticum were measured to determine their identity. Furthermore, the nucleotide sequences of three Stellaria species, S.vestita, S. longifolia and S.radians, were also measured for the sake of providing the evidence of the biological phylogeny of Stellaria. Diversity between DNA sequence and restriction enzyme mapping among a variety of the species was found in their 5S-rRNA spacer domains. Conclusion The 5S-rRNA spacer domains can be used as a molecular marker for differentiating S.media from M.aquaticum and in phylogenetic studies of Stellaria.