HPLC-ELSD法测定鸦胆子油中四种甘油三酯的含量

目的：建立鸦胆子油中1,2--二亚油酸-3-油酸甘油酯、1,3--二油酸-2-亚油酸甘油酯、三油酸甘油酯、1,3---油酸-2-棕榈甘油酯的含量测定方法.方法：运用岛津2010C高效液相色谱仪和奥泰2000蒸发光检测器,采用Waters C18色谱柱（250 mm×4.6mm,5μm）;流动相为乙腈-二氯甲烷（65∶35）,流速1.0 ml·min-1;检测温度：70℃.结果：四种甘油三酯分别在0.184～2.763 μg、0.382～5.724μg、0.406 ～6.084 μg和0.204～3.066 μg之间线性关系良好,r值分别为0.999 0、0.999 2、0.999 1和0.999 0,回收率分别为99.01％、98.70％、98.92％和98.46％,RSD分别为0.7％、0.5％、0.8％和0.9％.结论：该方法操作简单,分离效果好,灵敏度高,可更好的控制鸦胆子油的质量.

Determination of Four Kinds of Triglyceride in Brucea Javanica Oil by HPLC-ELSD

Objective：To establish an HPLC method for the determination of 1,2-dilinoleoyl-3-oleoyl-rac-glycerol,1,3-dioleoyl-2-linoleoyl-rac-glycerol,glycerol trioleate and 1,2-dioleoyl-3-palnitoyl-rac-glycerol in Brucea Javanica oil.Methods：The determination was performed using a Shimadzu 2010C HPLC with an Alltech2000 ESLD and a Waters C18 analytical column （250 mm ×4.6 mm,5 μm）,acetonitrile-dichloromethane was used as the mobile phase at the flow rate of 1.0 ml · min-1,the detection temperature was 70℃,and the column temperature was 30℃.Results：The linear range of four kinds of triglyceride was 0.184-2.763 μg,0.382-5.724 μg,0.406-6.084 μg and 0.204-3.066 μg （r =0.999 0,0.999 2,0.999 1 and 0.999 0）,and the average recovery was 99.01％,98.70％,98.92％ and 98.46％ with RSD of 0.7％,0.5％,0.8％ and 0.9％,respectively.Conclusion：The method is simple and sensitive with good separation efficiency,and it can be used in the quality control of Brucea Javanica oil.