正交试验法优选松茸多糖的提取工艺

目的：筛选松茸多糖的最佳提取条件。方法：用水提取松茸多糖，以浸提温度、浸提时间、料水比和浸提次数为主要因素，采用L9（3^4）正交试验进行提取工艺的优化；用苯酚一硫酸法测定多糖含量后计算多糖得率。结果：影响松茸多糖提取的主要因素由大到小依次为浸提时间〉浸提次数〉浸提温度〉料水比。结论：通过正交试验及其验证试验确定的松茸多搪的最佳提取条件为：浸提时间3h，浸提温度90℃，浸提次数2次，料水比1：30，在此条件下，松茸多糖得率可达12．24％。

Optimization on Extraction Conditions on Polysaccharide of Tricholoma matsutakes by Orthogonal Test

Objective： To screen the optimum conditions for extracting polysaccharide of T. matsutakes. Method： The polysaccharide of T. matsutakes was extracted with distilled water. The extraction temperature, extraction time, extraction ratio, extraction times were used as main factors, the L9 （3^4） orthogonal test was used for optimizing the extraction technology. The yield rate of polysaccharide was calculated after the polysaccharide content was detected by phenol-vitriol. Result： The main factors of effecting on the extraction for the polysaccharide of T. matsutakes from big to small were extraction time 〉 extraction times 〉 extraction temperature 〉 extraction ratio. Conclusion： The best conditions for extracting the polysaccharide by the orthogonal test and verification test was as follows： the extraction time for 3h,the extraction times for 2,the extraction temperature for 90℃, the extraction ratio for 1：30, and the yield of polysaccharide from T. matsutakes can achieve 12.24%.