| 人血管内皮抑素腺相关病毒载体包装质粒0pSNAV-hEndostatin-CMV-EGFP的构建 |
| |
| 引用本文： | 孙俊杰,尹利荣,糜若然.人血管内皮抑素腺相关病毒载体包装质粒0pSNAV-hEndostatin-CMV-EGFP的构建[J].天津医药,2009,37(7). |
| |
| 作者姓名： | 孙俊杰 尹利荣 糜若然 |
| |
| 作者单位： | 1. 天津医科大学第二医院妇科,300211
2. 天津医科大学总医院 |
| |
| 摘 要： | 目的:构建人血管内皮抑素(Endostatin)腺相关病毒(Adeno-associated virus,AAV)载体包装质粒pSNAV-hEndostatin-CMV-EGFP.方法:采用分子克隆技术,从pCD-sEndostatin质粒获得hEndostatin cDNA,并将PCR扩增产物插入至AAV包装质粒pSNAV上,构建pSNAV-hEndostatin-CMV-EGFP的AAV重组质粒,经PCR、酶切及测序鉴定.结果:经PCR、酶切鉴定及基因测序证实AAV包装质粒pSNAV-hEndostatin-CMV-EGFP构建成功.结论:构建的AAV包装质粒pSNAV-hEndostatin-CMV-EGFP可作为rAAV-hEndostatin-EGFP表达载体的包装质粒.
|
| 关 键 词： | 内皮抑素类 腺病毒科 质粒遗传 载体 |
Construction of Packaging Plasmid pSNAV-hEndostatin-CMV-EGFP of AAV Encoding Human Endostatin cDNA |
| |
| SUN Junjie,YIN Liming,MI Ruoran.Construction of Packaging Plasmid pSNAV-hEndostatin-CMV-EGFP of AAV Encoding Human Endostatin cDNA[J].Tianjin Medical Journal,2009,37(7). |
| |
| Authors: | SUN Junjie YIN Liming MI Ruoran |
| |
| Abstract: | Objective: To construct the packaging plasmid pSNAV-hEndostatin-CMV-EGFP of adeno-associated virus vector (AAV) encoding human Endostatin (hEndostatin) cDNA. Methods: The hEndostatin eDNA obtained from plasmid pCD-sEndostatin was subcloned into the packaging plasmid pSNAV of AAV by molecular clone ways. The recombinant plasmid pSNAV-hEndostatin-CMV-EGFP was identified by PCR analysis, restriction enzymes analysis and sequencing analysis.Results: The recombinant pSNAV-hEndostatin-CMV-EGFP was correctly constructed and confirmed by PCR analysis, restriction enzymes analysis and sequencing analysis. Conclusion: The constructed AAV-hEndostatin packaging plasmid pSNAV-hEndostatin-CMV-EGFP could be the packaging plasmid of rAAV-hEndostatin-EGFP. |
| |
| Keywords: | endostatins adenoviridae plasmids genetic vectors |
| 本文献已被 万方数据 等数据库收录！ |
