Livin和Smac在膀胱移行细胞癌中的表达及相关性研究

目的:探讨Livin蛋白和Smac蛋白在膀胱移行细胞癌(BTCC)中的表达及两者的相关性.方法:应用免疫组化SABC法检测42例BTCC组织和18例癌旁正常黏膜组织中Livin蛋白和Smac蛋白的表达情况,并分析两者与BTCC临床病理特征的关系.结果:Livin蛋白和Smac蛋白在BTCC组织中的阳性表达率分别73.8％(31/42)和28.6％(12/42),而在18例癌旁正常黏膜组织中分别为0和77.8％(14/18),两者在癌和癌旁间的表达差异均有统计学意义(P＜0.01)；在有无复发的患者中Livin蛋白和Smac蛋白的表达差异均有统计学意义(P＜0.05),不同性别、年龄、组织学分级、临床分期、肿瘤数目Livin蛋白和Smac蛋白在BTCC中的表达差异均无统计学意义.Livin蛋白和Smac蛋白在BTCC组织中的表达呈负相关(r=-0.462,P＜0.05).结论:Livin蛋白高表达和Smac蛋白低表达或缺失表达可能是导致BTCC的发生及复发的原因,两者可能是BTCC细胞凋亡信号传导网络中的重要一环.

Correlation of Livin and Smac Expression in Bladder Transitional Cell Carcinoma

Objective: To investigate the expression of Livin protein and second mitochondria-derived activator of caspases (Smac) protein in the bladder transitional cell carcinoma (BTCC), and their correlation thereof. Methods:The expression levels of Livin and Smac proteins were assessed in 42 tissue samples of BTCC and 18 samples of adjacent normal mucosa by SABC immunohistochemistry, and their relationships with clinical pathological features were analyzed. Results: The positive expression rates of Livin and Smac proteins in BTCC were 73.8% (31/42) and 28.6% (12/42), while they were 0 and 77.8% (14/18) in 18 samples of adjacent normal mucosa. The differences in expressions of Livin and Smac proteins were significant between BTCC and adjacent normal mucosa (P < 0.01). There were significant differences in expressions of Livin and Smac proteins between patients with and without recurrence (P < 0.05). There were no significant differences between gender, age, histological grades, clinical stages, the number of tumors and expressions of Livin and Smac proteins in BTCC. There was a negative correlation between the expression levels of Livin proteins and Smac proteins in BTCC (rs = -0.462, P < 0.05). Conclusion: The relatively higher expression of Livin protein and the lower or not expression of Smac protein may lead to the occurrence and recurrence of the BTCC, and both maybe play an important role in the cell apoptosis of the BTCC.