夏枯草硫酸多糖对四氯化碳致大鼠肝纤维化的干预作用

摘要： 目的 探讨夏枯草硫酸多糖 （PVSP） 对四氯化碳 （CCl4 ） 致大鼠纤维化的干预作用。方法 采用 CCl4-橄榄油腹腔注射诱导建立 SD 大鼠肝纤维化模型， 将造模成功的大鼠随机分成 3 组， 每组 10 只， 分别为模型组 （Model 组）、 PVSP 高剂量组 （PVSP-H 组： 400 mg/kg） 和 PVSP 低剂量组 （PVSP-L 组： 100 mg/kg）。并设空白对照组 （Blank 组） 和溶剂对照组 （Solvent 组）。采用全自动生化分析仪测定血清丙氨酸转氨酶 （ALT） 和天冬氨酸转氨酶 （AST） 的含量， HE 和天狼猩红染色比较炎症及肝纤维化程度； 采用 qRT-PCR 和免疫组织化学分析肝组织中Ⅰ型胶原 （Col- Ⅰ）、 平滑肌肌动蛋白 （α-SMA） mRNA 和蛋白的表达量。结果 Solvent 组大鼠血清中 ALT、 AST 及肝组织中 Col-Ⅰ、 α-SMA mRNA 和蛋白与 Blank 组相比差异均无统计学意义； 与 Model 组相比， PVSP 能显著降低血清 ALT 和 AST （P＜0.05）； HE 和天狼猩红染色结果显示 PVSP 能减轻炎症及纤维化程度； qRT-PCR 和免疫组织化学结果显示 PVSP 明显降低大鼠肝脏内 Col-Ⅰ、 α-SMA mRNA 和蛋白表达 （P＜0.05）。结论 PVSP 具有减轻大鼠肝纤维化作用， 其机制可能与抑制Col-Ⅰ、 α-SMA 表达， 减少细胞外基质的生成并促进细胞外基质的降解有关。

Interventional effects of prunella vulgaris sulfated polysaccharide on CCl4-induced hepatic fibrosis in rats

Abstract: Objective To investigate the intervention effects of prunella vulgaris sulfated polysaccharide (PVSP) on carbon tetrachaloride (CCl4)-induced hepatic fibrosis in rat. Methods The 40% CCl4 was used to induce hepatic fibrosis in rat model, then successful model rats were randomly divided into 3 groups, with 10 rats in each group, respectively, the model group (Model), the high dose PVSP group (PVSP-H: 400 mg/kg) and the low dose PVSP group (PVSP-L: 100 mg/kg). The blank group and solvent group were also established. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by automatic biochemical analyzer. HE staining and Sirius red staining were used to examine the degree of hepatic fibrosis. The expression levels of collagen type Ⅰ (Col-Ⅰ) and α - smooth muscle actin (α- SMA) mRNA were detected by qRT-PCR. Col-Ⅰ and α-SMA in hepatic tissues were detected by immunohistochemistry staining. Results There were no significant changes in serum expressions of ALT and AST and mRNA proteins of Col-Ⅰ and α-SMA in liver tissues between the blank group and Solvent group. Compared with the model group, the serum levels of ALT and AST were significantly decreased in the PVSP-H and PVSP-L groups (P＜0.05). HE staining and Sirius red staining showed that PVSP could significantly reduce the degree of hepatic fibrosis. The expression of Col-Ⅰ and α-SMA mRNA were decreased in the PVSP-H and PVSP-L groups (P＜0.05). Immunohistochemistry showed that the expressions of Col - Ⅰ and α - SMA in hepatic tissues were decreased by PVSP (P＜0.05), and the effect was dose-dependent. Conclusion PVSP has a protective effect on CCl4 - induced hepatic fibrosis in rats, which may be related with the inhibiting expressions of Col-Ⅰand α-SMA, reducing secretion of collagen and promoting extracellular matrix degradation.