替米沙坦对2型糖尿病大鼠肾脏内氧化立激及NIH3T3细胞中促成熟因子活性的影响

目的探讨替米沙坦对2型糖尿病大鼠肾脏氧化应激和NIH3T3细胞中促成熟因子（MPF）活性的影响。方法将动物分为正常对照组、糖尿病组及替米沙坦治疗组，检测各组给药4，5，11，17周后的血糖、血胰岛素、血脂（TG，TC），11，17周的血肌酐（Scr）、血尿素氮（BUN）、尿微白蛋白排泄率（UAE）、肾组织中丙二醛（MDA）含量、铜，锌-超氧化物歧化酶（Cu，Zn—SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化酶（GSH—Px）及MPF活性。结果与糖尿病组相比，替米沙坦治疗组血糖、血胰岛素及血脂无明显变化，而Scr，BUN，UAE、肾脏MDA含量、肾细胞膜MPF均明显下降，肾脏抗氧化酶活性（Cu，Zn—SOD，CAT，GSH—Px）则明显上升。肾脏内MDA含量的变化及抗氧化酶活性的变化与细胞膜、细胞浆MPF活性的变化相关。结论替米沙坦可以抑制2型糖尿病大鼠肾脏内的氧化应激，并且可能与其下调NIH3T3细胞中MPF活性有关。

Influence of Telmisartan on Oxidative Stress and Activity of Maturation Promoting Factor in NIH3T3 Cells on Type 2 Diabetic Rat

Objective To observe the influence of telmisartan on oxidative stress and the activity of maturation promoting factor in type 2 diabetic rat.Methods Thirty-six Wistar rats were divided into three groups of normal rats,diabetic rats and diabetic rats treated with telmisartan.Blood glucose,blood insulin,blood lipid were measured after 4,5,11,17 weeks,serum creatinine,blood uria nitrogen,urinary albumin excretory rate,lipid peroxidation(MDA),the activity of maturation promoting factor (MPF) and of antioxidant enzymes including Cu/Zn superoxide dismutase(Cu,Zn-SOD),catalase(CAT) and glutathione peroxidase(GSH-Px) in renal tissue were measured after 11 and 17 weeks.In addition,the renal tissues were observed by light microscopy.Results The blood glucose and blood insulin were found no significant difference between those in type 2 diabetic rats with and without telmisartan treatment groups.Comparing with the untreated group,the level of blood lipid,serum creatinine,blood uria nitrogen,urinary albumin excretory rate,renal MDA and the activity of cell membrane MPF in telmisartan treated group were significantly decreased,but the activity of renal Cu/Zn SOD,CAT and GSH-Px was increased.There was significant correlation between the change of renal MDA and the activity of antioxidase and the activity of cell membrane MPF.Conclusion Telmisartan may inhibit oxidative stress in diabetic rats,and its mechanism perhaps is decreasing the activity of cell membrane MPF.