| 4个黄芪异黄酮类化合物对PC12细胞分化的影响 |
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| 引用本文： | 吴丘玲,成彦琼,刘爱军,张卫东.4个黄芪异黄酮类化合物对PC12细胞分化的影响[J].药学实践杂志,2020,38(3):232-236. |
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| 作者姓名： | 吴丘玲 成彦琼 刘爱军 张卫东 |
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| 作者单位： | 福建中医药大学药学院, 福建 福州 350122;海军军医大学药学院药理学教研室, 上海 200433;福建中医药大学药学院, 福建 福州 350122;海军军医大学药学院天然药物化学教研室, 上海 200433 |
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| 摘 要： | 目的探究毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷对PC 12细胞分化的影响。方法培养PC 12细胞,与不同浓度的神经生长因子(NGF)、毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷共处理5 d,1次/d,连续3次,观察PC 12细胞突起向外生长的情况。同时,用免疫荧光染色检测上述化合物对β微管蛋白(βⅢ-tubulin)表达的影响。结果与对照组相比,毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷和芒柄花苷(0.01~10.00μmol/L)未能促进PC 12细胞突起向外生长和β微管蛋白的表达。结论毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷不能促进PC 12细胞分化。
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| 关 键 词： | 毛蕊异黄酮 刺芒柄花素 毛蕊异黄酮苷 芒柄花苷 PC 12细胞 细胞分化 |
| 收稿时间： | 2020/1/10 0:00:00 |
| 修稿时间： | 2020/3/6 0:00:00 |
The effect of four compounds of astragalus isoflavones on the differentiation of PC 12 cells |
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| WU Qiuling,CHENG Yanqiong,LIU Aijun,ZHANG Weidong.The effect of four compounds of astragalus isoflavones on the differentiation of PC 12 cells[J].The Journal of Pharmaceutical Practice,2020,38(3):232-236. |
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| Authors: | WU Qiuling CHENG Yanqiong LIU Aijun ZHANG Weidong |
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| Affiliation: | School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;Department of Pharmacology, School of Pharmacy, Naval Medical University, Shanghai 200433, China; School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China;Department of Natural Medicinal Chemistry, School of Pharmacy, Naval Medical University, Shanghai 200433, China |
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| Abstract: | Objective To investigate the effects of calycosin, formononetin, calycosin-7-glucoside and ononin on PC 12 cells differentiation. Methods PC 12 cells were cultured and treated with different concentrations of nerve growth factor(NGF),calycosin, formononetin, calycosin-7-glucoside and ononin for 5 days, once a day, 3 times in a row. The neurite outgrowth of PC 12 cells was observed and the expression of β Ⅲ-tubulin were measured by immunofluorescence. Results Compared with the vehicle group, neurite outgrowth and the expression of β Ⅲ tubulin in PC 12 cells had not promoted by calycosin, formononetin, calycosin-7-glucoside and ononin(0.01-10.00 μmol/L). Conclusion PC 12 cells differentiation could not be induced by calycosin,formononetin, calycosin-7-glucoside and ononin. |
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| Keywords: | calycosin formononetin calycosin-7-glucoside ononin PC 12 cells cell differentiation |
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