7种美白化合物对皮肤黑素细胞的体外生物学效应

目的 研究7种美白化合物对体外培养的皮肤黑素细胞的生物学效应。方法 利用B-16小鼠黑素瘤细胞株,以左旋多巴为底物测定细胞内酪氨酸酶活性,采用比色法测定细胞中黑色素含量,以溴化二苯四偶氮法（MTT法）测定化合物对黑素细胞活力的影响。结果 各化合物对黑素细胞酪氨酸酶活性和黑素生成量呈浓度依赖性抑制,对黑素细胞活力均有不同程度的影响。结论 这7种化合物均能抑制黑素细胞的黑色素生成。

Bio-efficacy of seven skin-lighteners on dermal melanocytes in vitro

Objective To investigate the bio-efficacy of seven skin-lighteners on melanocytes in vitro. Methods After B-16 melanoma cells were treated with skin-lightener, the tyrosinase activity, the melanin content and cell viability were measured by utilization of L-Dopa as the substrate, colorimetric analysis, and MTT assay, respectively. Results each of the skin-lighteners showed a concentration-dependent reduction in tyrosinase activity and the melanin content and partially inhibited the cell viability. Conclusion all of the seven skin-lighteners could inhibit the melanocytes to produce melanin.