| 酪氨酸酶基因工程菌产黑色素的发酵条件研究 |
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| 引用本文： | 王戈林,宁华.酪氨酸酶基因工程菌产黑色素的发酵条件研究[J].中国医药工业杂志,1999,30(4):150-154. |
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| 作者姓名： | 王戈林 宁华 |
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| 作者单位： | 武汉大学生命科学学院!武汉430072 |
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| 摘 要： | 研究了表达酪氨酸酶的基因工程菌产黑色素的条件。ＷＹ８能在酪素平板上产生可溶性黑色素，在添加酪氨酸的液体ＬＢ培养基中的黑色素产率是基因来源菌－Ｐｓｅｕｄｏｍｏｎａｓ ｍａｌｔｏｐｈｉｌｉａ的５倍。用正交试验法找到适宜发酵产黑色素的培养基，对摇瓶装置，菌龄，底物浓度，发酵温度和ｐＨ等因素的影响作了进一步探讨。
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| 关 键 词： | 工程菌 酪氨酸酶 黑色素 发酵条件 |
STUDY ON PRODUCTION OF MELANIN BY TYROSINASE GENE ENGINEERING BACTERIA |
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| Authors: | WANG Ge Lin NING Hua SHEN Ping PENG Zhen Rong |
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| Abstract: | The condition for the production of melanin by engineering bacteria (E.coli WY8) bearing tyrosinase gene was examined.WY8 could form soluble melanin pigments on the casein agar plates.Its melanin productivity was 5 times as much as the bacteria of tyrosinase gene source (Pseudomonas maltophilia) in liquid LB culture supplemented with tyrosine.By means of orthogonal transformation the suitable medium for melanin fermentation was obtained.The effects of flask volume,culture age,substrate concentration,fermentation temperature and pH were also studied. |
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| Keywords: | engineering bacteria tyrosinase melanin |
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