错配修复和微卫星不稳定性与食管癌的关系

目的探讨DNA错配修复基因、微卫星不稳定性与食管癌发生发展的关系。方法采用放射性同位素为基础的聚合酶链式反应(PCR)技术,对96例食管癌中错配修复基因和4个位点微卫星不稳定性进行了检测。结果 96例食管癌中hmsh3、hmsh6基因突变率分别为10.4%和25%,4个位点微卫星不稳定性阳性率分别为D2S123(12.5%)、BAT-26(18.8%)、D17S261(10.4%)、D17S799(8.3%),hmsh3、hmsh6基因突变和微卫星不稳定性多为分化不良的癌,而与患者的性别、淋巴结转移无关。结论错配修复基因突变与微卫星不稳定性是食管癌发生的早期分子事件,是除LOH致癌途径以外的又一新的致癌途径。

Clinical Analysis of Relationship about Mismatch Repair, Microsatellite Instability and Esophageal Cancer

Objective To observe the relationship between the genes of DNA mismatch repair and Genomics instability and the esophageal cancer＇s development.Methods Using the PCR to test the genes of DNA mismatch repair and 4 Genomics instability sites in tumor tissue of 48 patients.Results The rate of gene mutation of hmsh3,hmsh6 are 10.4% and 25%,resectively.The positive of 4 sits of the micsatellite the instability are D2S123（12.5%）,BAT-26（18.8%）,D17S261（10.4%）,D17S799（8.3%）,resectively.The mutantiongenes of hmsh3,hmsh6 and Genomics instability were relation to the poor differentiation carcinoma,and independent of the gender and the lymph node transfer.Conclusion DNA mismatch repair and Genomics instability were happen in the early stage of esophageal cancer.They were another way to oncogenic the esophageal cancer,except the LOH way.