T-226296: a novel, orally active and selective melanin-concentrating hormone receptor antagonist |
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Authors: | Takekawa Shiro Asami Asano Ishihara Yuji Terauchi Jun Kato Kaneyoshi Shimomura Yukio Mori Masaaki Murakoshi Hitomi Kato Koki Suzuki Nobuhiro Nishimura Osamu Fujino Masahiko |
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Affiliation: | Discovery Research Laboratories I, Pharmaceutical Research Division, Takeda Chemical Industries, Ltd., Wadai 10, Ibaraki 300-4293, Tsukuba, Japan. |
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Abstract: | Through the screening of our in-house chemical compound library, we found a novel melanin-concentrating hormone (MCH) receptor antagonist, T-226296, a (-) enantiomer of N-[6-(dimethylamino)-methyl]-5,6,7,8-tetrahydro-2-naphthalenyl]-4'-fluoro[1,1'-biphenyl]-4-carboxamide. T-226296 exhibited high affinity for cloned human and rat MCH receptors (SLC-1) in receptor binding assays (IC50=5.5+/-0.12 nM for human SLC-1; 8.6+/-0.32 nM for rat SLC-1). T-226296 had high selectivity over other receptors, including the second subtype of the MCH receptor, SLT (MCH2), transporters and ion channels. In Chinese hamster ovary (CHO) cells expressing human SLC-1, T-226296 reversed the MCH-mediated inhibition of forskolin-stimulated cAMP accumulation, inhibited MCH-induced intracellular Ca2+ increase, and also inhibited MCH-stimulated arachidonic acid release. In rats, oral administration of T-226296 (30 mg/kg) almost completely suppressed the food intake induced by intracerebroventricular injection of MCH. These results clearly indicate that T-226296 is a novel, orally active and selective MCH receptor antagonist that will be promising for further exploring the physiology and pathophysiology of MCH-SLC-1 signaling. |
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Keywords: | (MCH) melanin-concentrating hormone SLC-1 receptor Receptor antagonist Food intake Obesity |
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