某市嘉陵江水中提取物对大鼠肝细胞AHR信号途径的诱导

目的研究某市主城区嘉陵江水中有机污染物对大鼠肝细胞细胞色素P450 1A1(cytochrome P450 1A1,CYP1A1)基因表达和7-乙氧基-3-异吩嗯唑酮.脱乙基酶(7-ethoxyresorufin-O-deethylase,EROD)活力的诱导,比较不同剂量的有机提取物诱导大鼠肝细胞CYP1A1基因表达量和EROD酶活力的变化。方法将大鼠分为2、12和72 L/(kg体重)3个染毒组及1个玉米油溶剂对照组和1个空白对照组,每组20只动物,雌雄各半,灌胃染毒13周。染毒结束,提取大鼠肝细胞总RNA进行CYP1A1基因的RT-PCR扩增,将肝组织匀浆后测定其EROD酶活力。结果各染毒剂量组CYP1A1基因均有表达,空白对照和溶剂对照组CYP1A1基因不表达;中剂量组与高剂量组CYP1A1的相对表达量与低计量组相比,增加非常显著(P<0.01),各染毒组大鼠肝组织均可检出EROD酶活力,EROD酶活力随染毒剂量的增加而增加。结论嘉陵江水中有机污染物可诱导大鼠肝细胞芳烃受体(aryl hydrocarbon receptor,AHR)途径。

The inducement of AHR signal pathway in rat hepatocytes of the organic extraction from Jialing river of chongqing city

Objective To detect the inducement of cytochrome P450 1A1(CYP1A1) mRNA and 7-ethoxyresorufin-O-deethylase(EROD) activity in rat hepatocytes of the organic pollutants in Jialing river of chongqing city,and to compare the mRNA quantities and enzyme activity among different dose groups.Method 100 male and female rats were equally divided into three dose groups of 2,12,72 L/kg body weight(BW),a solvent control group,and a blank control group.The organic extracts were poured into stomachs every two days for 13 weeks.After exposure,the total RNA was extracted and amplified the CYP1A1 gene using RT-PCR,the liver was slurried and the EROD activity was detected.Results CYP1A1 gene was expressed in each dose groups,but not in the control groups,and the relative expression quantities of middle dose group and high dose group were very significantly higher than that of the low dose group;the EROD activity could be detected in each dose group and the enzyme activity was increased with the increase of the dose.Conclusion The rat hepatocytes aryl hydrocarbon receptor(AHR)signal pathway could be induced by the organic pollution in Jialing River.