益气养阴合剂中10种有效成分的含量测定

目的:建立同时测定益气养阴合剂中丹参酮ⅡA、丹酚酸A、丹酚酸B、毛蕊异黄酮、毛蕊异黄酮葡萄糖苷、五味子醇甲、五味子醇乙、五味子甲素、五味子乙素、丹皮酚含量的方法。方法:采用Hedera C18 ODS-2色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸水溶液梯度为流动相,柱温30℃,流速1.0 mL·min^-1,检测波长215nm。结果:毛蕊异黄酮、毛蕊异黄酮葡萄糖苷、丹皮酚、五味子醇甲、五味子醇乙、五味子甲素、五味子乙素、丹参酮ⅡA、丹酚酸A、丹酚酸B浓度分别在2.93~62.76(r=0.999 6)、2.53~54.06(r=0.999 9)、2.78~59.64(r=0.999 9)、2.72~58.38(r=0.999 2)、2.79~59.76(r=0.999 6)、2.87~61.44(r=0.999 7)、2.61~63.66(r=0.999 2)、2.28~48.78(r=0.999 6)、2.99~64.08(r=0.999 5)、2.69~57.66(r=0.999 1)范围内与峰面积呈良好的线性关系,平均回收率在93.23%~100.68%之间。结论:3批样品测定结果表明,建立的方法可以用于同时测定益气养阴合剂中10种成分的含量。

Determination of 10 active components in Yiqi Yangyin mixture

OBJECTIVE To establish an HPLC method for simultaneous determination of tanshinone ⅡA, salvianolic acid A, salvianolic acid B, granule Isoflavones, myrone isoflavones glucoside, schisandrin A, schisandrin B,schisandrin, schisandrin and paeonol content. METHODS HPLC separation was carried out on Hedera C₁₈ ODS-2(4.6 mm×250 mm,5 μm)column with a gradient elution composed of acetonitrile and 0.1% phosphoric acid solution at 30℃ at a flow rate of 1.0 mL·min^-1 and the detection wavelength of was 215 nm.RESULTS The linear range of calycosin, calycosin-7-glucoside, paeonol, schisandrin, schisandrol B, deoxyschlizandrin, schizandrin B, tanshinone ⅡA,salvianolic acid A,salvianolic acid B were 2.93-62.76(r=0.999 6),2.53 -54.06(r=0.999 9),2.78-59.64(r=0.999 9),2.72-58.38(r=0.999 2),2.79-59.76(r=0.999 6),2.87-61.44(r=0.999 7),2.61-63.66(r=0.999 2),2.28-48.78(r=0.999 6),2.99-64.08(r=0.999 5),2.69-57.66(r=0.999 1) and the peak area showed a good linear relationship with average recoveriesy rates of 93.23%-100.68%. CONCLUSION The results of three batches of samples showed that the established method could be used for simultaneous determination of 10 components in Yiqi Yangyin mixture.