| 疏血通脉胶囊定性定量方法研究 |
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| 引用本文： | 兰保强,;刘泰,;伍小燕,;曹斌,;饶伟源,;邓聿胤.疏血通脉胶囊定性定量方法研究[J].中南药学,2014(9):899-902. |
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| 作者姓名： | 兰保强 ;刘泰 ;伍小燕 ;曹斌 ;饶伟源 ;邓聿胤 |
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| 作者单位： | [1]广西中医药研究院,南宁530022; [2]广西中医药大学第一附属医院,南宁530023; [3]广西中药质量标准研究重点实验室,南宁530022 |
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| 基金项目： | 广西科学研究与技术开发计划项目（编号：桂科攻11107009-1-11） |
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| 摘 要： | 目的建立疏血通脉胶囊定性定量研究方法。方法采用TLC法对制剂中的薤白、瓜蒌皮、冰片进行鉴别,采用HPLC法,对制剂中的人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1进行含量测定。结果 TLC法可检出薤白、瓜蒌皮、冰片。人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1的线性范围分别为0.1021.020、0.0951.020、0.0950.950和0.0350.950和0.0350.350 mg·mL-1(r=0.999 9),平均回收率分别为98.9%、99.6%和100.4%,RSD分别为2.0%、1.7%和1.7%(n=6)。结论该方法操作简单可靠,专属性强,可用于控制疏血通脉胶囊的质量。
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| 关 键 词： | 疏血通脉胶囊 定性定量方法 薄层色谱法 高效液相色谱法 |
Quantitative and qualitative method for Shuxue Tongmai capsules |
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| Affiliation: | LAN Bao-qiang, LIU Tai, WU Xiao-yan, CAO Bin, RAO Wei-yuan, DENG Yu-yin (1. Guangxi Academy of Traditional Chinese Medical and Pharmaceutical Sciences, Nanning 530022; 2. The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023; 3. Guangxi Key Laboratory of Quality Stardands of Tradtitional Chinese Medicine, Nanning 530022) |
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| Abstract: | Objective To establish the quantitative and qualitative method for Shuxue Tongmai capsules. Methods TLC was adopted to identify Allium macrosternon Bunge, Trichosanthis Pericarpium and Borneol, and HPLC was ad- opted to determine the content of ginsnoside Rg1, ginsnoside Rb1 and notoginsenoside R1 in Shuxue Tongmai capsules. Results Allium macrostemon Bunge, Trichosanthis Pericarpium and Borneol were identified by TLC. The linear range was 0.102 - 1.020 mg · mL-1 for ginsenoside Rg1, 0.095 - 0.950 mg ·mL -1 for ginsenoside Rb1, and 0.035 -0.350 mg · mL 1 for notoginsenoside Rt （r = 0.999 9）. The average recovery was 98.9%, 99.6% and 100.4%, re- spectively, with the RSD of 2.0%, 1.7% and 1.7%. Conclusion The method is simple and rapid, which is suitable for the quality control of Shuxue Tongmai capsules. |
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| Keywords: | Shuxue Tongmai capsule quantitative and qualitative method TLC HPLC |
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