| 5SrRNA基因间隔区DNA序列在穿心莲道地性中的可行性研究 |
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| 引用本文： | 李劲平,王培训,曹柳英,梁瑞叶,张洪建.5SrRNA基因间隔区DNA序列在穿心莲道地性中的可行性研究[J].中南药学,2007,5(5):438-440. |
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| 作者姓名： | 李劲平 王培训 曹柳英 梁瑞叶 张洪建 |
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| 作者单位： | 中南大学药学院 长沙410013(李劲平，张洪建)，广州中医药大学 广州510405(王培训，曹柳英，梁瑞叶) |
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| 摘 要： | 目的探讨5SrRNA基因间隔区DNA序列在穿心莲道地性研究中的可行性。方法提取不同产地穿心莲总DNA后,PCR特异扩增5SrRNA基因间隔区,采用荧光标记末端终止子双脱氧末端终止法测序,对测序结果进行多序列联配分析。结果成功得到321bp的5SrRNA基因间隔区碱基序列,经过多序列联配分析发现,不同产地穿心莲的此段5SrRNA基因间隔区DNA序列之间没有差异。结论5SrRNA基因间隔区DNA序列不适合用于研究穿心莲药材的道地性。
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| 关 键 词： | 穿心莲 5SrRNA基因间隔区 DNA测序 |
| 文章编号： | 1672-2981（2007）05-0438-03 |
| 修稿时间： | 2007-04-18 |
Feasibility of the DNA sequence to internal transcribed spacer regions of 5SrRNA gene to the genuineness of Andrographis paniculata(Burm.f.)Ness. |
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| LI Jin-ping,WANG Pei-xun,CAO Liu-ying,LIANG Rui-ye,ZHANG Hong-jian.Feasibility of the DNA sequence to internal transcribed spacer regions of 5SrRNA gene to the genuineness of Andrographis paniculata(Burm.f.)Ness.[J].Central South Pharmacy,2007,5(5):438-440. |
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| Authors: | LI Jin-ping WANG Pei-xun CAO Liu-ying LIANG Rui-ye ZHANG Hong-jian |
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| Affiliation: | 1. School of Pharmaceutical Sci- ences, Central South University, Changsha 410013; 2. Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405 |
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| Abstract: | Objective To determine the feasibility of the DNA sequence to the internal transcribed spacer regions of 5SrRNA gene to the genuineness of Andrographis paniculata(Burm.f.) Ness.Methods We extracted the total DNA from the leaves of Andrographis paniculata(Burm.f.) Ness.by conventional method,then used the peculiar primers to amplify the internal transcribed spacer regions of 5SrRNA gene,and measured the base sequence of the amplified products.Results There was no polymorphism in the internal transcribed spacer regions of 5SrRNA gene between Andrographis paniculata(Burm.f.) Ness from several regions.Conclusion The DNA sequence of internal transcribed spacer regions of 5SrRNA gene has no value for the genuineness of Andrographis paniculata(Burm.f.) Ness. |
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| Keywords: | andrographitis internal transcribed spacer regions of 5SrRNA gene DNA sequencing |
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