响应面法优化链霉菌HY6-S36产星孢菌素的发酵条件

摘要：目的 优化链霉菌HY6-S36产星孢菌素的发酵条件，以提高星孢菌素的产量。方法 在单因素试验的基础上通过 Plackett-Burman试验、最陡爬坡试验、Box-benhnken(BB)响应面法优化发酵条件。结果 优化后，最佳培养条件为麦芽糊精 69.8g/L，豆粉49.6g/L，烟酸0.5g/L，碳酸钙4.0g/L，转速200r/min，pH7.2，装液量30mL/250mL，接种量5%，温度28℃，发酵 时间7d，摇瓶产量为996mg/L。经50L发酵罐放大培养，发酵效价达1063mg/L。结论 在此优化条件下，星孢菌素的摇瓶产量 提高了177%。

Optimization fermentation conditions of staursporine from Streptomyces sp. HY6-S36 by response surface methodology

Abstract Objective The fermentation conditions were optimized for Streptomyces sp. HY6-S36 to improve staursporine titer. Methods The fermentation conditions were tested by single-factor experiment, Plackett- Burman experiment, the steepest ascent path and Box Benhnken (BB) response surface method. Results After optimization, the optimal culture conditions were maltodextrin 69.8g/L, soybean powder 49.6g/L, nicotinic acid 0.5g/L, calcium carbonate 4.0g/L; rotating speed 200r/min, pH7.2, broth content 30mL/250mL, inoculum size 5%, culture temperature 28℃, fermentation period 7d, and the optimum yield was 996mg/L. Under the optimal 50L fermenter, the staurosporine production was 1,063mg/L. Conclusion Under optimal conditions, the shake flask of staurosporine production increased by 177%.