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| 在新分离的稀有放线菌群体中检测线型染色体、质粒和噬菌体 | |
| 引用本文： | 田永强,褚以文,赵国屏,覃重军.在新分离的稀有放线菌群体中检测线型染色体、质粒和噬菌体[J].中国抗生素杂志,2006,31(7):403-405. |
| 作者姓名： | 田永强 褚以文 赵国屏 覃重军 |
| 作者单位： | 1. 中国科学院上海生命科学院植物生理生态研究所,上海,200032;四川大学化学工程学院制药与生物工程系,成都,610065 2. 中国医药集团四川抗菌素工业研究所,成都,610051 3. 中国科学院上海生命科学院植物生理生态研究所,上海,200032 |
| 基金项目： | 国家高技术研究发展计划(863计划);国家自然科学基金 |
| 摘 要： | 稀有放线菌属于革兰阳性细菌，具有重要的应用价值。目前对绝大多数稀有放线菌还没有合适的遗传操作系统。本文报道利用脉冲电泳技术和碱变性方法，对土壤中新分离的约200株稀有放线菌的遗传物质进行了检测。结果显示，除约20个菌株的染色体可能为环型结构外，其余染色体为线型结构。在检测到的染色体外遗传因子中，包括在诺卡菌属菌株中检测到1个线型质粒、4个环型质粒和1个噬菌体，此外，在链孢囊菌属中首次检测到1个质粒。这些遗传因子的发现，为发展稀有放线菌类群的遗传操作体系提供了基础。 |
| 关 键 词： | 稀有放线菌 染色体 质粒 噬菌体 |
| 文章编号： | 1001-8689(2006)07-0403-03 |
| 收稿时间： | 2006-02-03 |
| 修稿时间： | 2006-04-14 |
Detection of the linear chromosomes, plasmids and phage among a population of the newly isolated rare-actinomycetes strains |
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| Tian Yong-qiang,Chu Yi-wen,Zhao Guo-ping,Qin Zhong-jun.Detection of the linear chromosomes, plasmids and phage among a population of the newly isolated rare-actinomycetes strains[J].Chinese Journal of Antibiotics,2006,31(7):403-405. | |
| Authors: | Tian Yong-qiang Chu Yi-wen Zhao Guo-ping Qin Zhong-jun |
| Affiliation: | 1 Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032; 2 School of Chemical Engineering, Sichuan University, Chengdu 610065; 3 Sichuan Industrial Institute of Antibiotics, China National Pharmaceutical Group Corp., Chengdu 610051 |
| Abstract: | The rare actinomycetes are Gram-positive bacteria of significant applications, however, a few of genetic manipulation systems available for them. This paper reports detection of the genetic elements by using the methods of pulsed-field gel electrophoresis and alkaline denaturation among the newly isolated 200 rare-actinomycetes strains. The results showed about 180 strains contained chromosomes in linear form, while the other 20 in circular form. One linear plasmid, four circular plasmids and one phage were detected in Nocardia strains, and one circular plasmid was first detected in Streptosporangium. Discovery of these genetic elements provide a basis for developing gene manipulation systems in rare actinomycetes. |
| Keywords: | Rare actinomycetes Chromosome Plasmid Phage |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
