螺旋链霉菌溶源性菌株的确证及生物学特性

用螺旋链霉菌噬菌体1010、1012、P11、4031分别感染S.spiramyceticus n.sp.298-36,培养后经分离得到了四株不同品系的溶源菌,并且对其生物学特性进行了研究。经多次单菌落分离试验及用抗血清处理溶源菌菌丝,在培养过程中发现这些菌株仍具有释放噬菌体的能力,并对其自身释放的噬菌体具有免疫性。溶源菌所释放的噬菌体经增殖后抽提DNA并酶切和电泳,结果表明这些噬菌体DNA的酶切位点及片段大小与相应的用于溶源化的噬菌体DNA的酶切位点及片段大小完全相同。溶源菌合成螺旋霉素的能力发生了很大的变化。用紫外线或丝裂霉素C诱导噬菌体P11的溶源化菌株,结果表明噬菌体的释放量没有显著增加。

IDENTIFICATION AND BIOLOGICAL FEATURES OF LYSOGENIC STRAINS OF STREPTOMYCES SPIRAMYCET1CUS N.SP.298-36

Using 5. spiramyceticus phage 1010, 1012, Pll and 4031 infect S. spiramyceticus n. sp. 298-36 respectively. After culture, four variant lysogenic strains were obtained, and their biological features were studied. By isolation of single colony for several times, the lysogenic strains were still able to release phages. When the mycelia of each lysogenic strain were treated with antiserum of corresponding phage, it was found that the strains could release phages continuously. Every lysogenic strain showed immunity to phage released. DNA of phages to be released by each lysogenic strain were extracted and then digested with restriction endonudease. It was fouad that the cleavage sites and restriction fragment length of the DNA of phages to be released by lysogenic strain are the same as that of the original phage. Great changes of ability of spiramycin synthesis take place in lysogenic strains. Induce reaction of ultraviolet light and mitomycin C were not obvious in the strain which lysogenized with phage P11.