绛红小单孢菌突变株CH20190225-107的选育与鉴定

摘要：目的 高产庆大霉素产生菌的筛选。方法 对出发菌株绛红小单孢菌GM20190109-15进行常温等离子体(ARTP)和氯化锂复合诱变处理。结果 经高通量筛选，得到一株突变菌株CH20190225-107，经高效液相色谱检测，突变菌株的各组份出峰时间与对照一致，其效价从1547 U/mL提高到2280 U/mL，摇瓶效价较出发菌株提高了47.4%，组分C1、C1a、C2a和C2的相对比例分别为29%、23%、25%和23%，且遗传性状稳定。结论 使用ARTP和氯化锂复合诱变庆大霉素产生菌的，效果较好，提高了庆大霉素的产量和质量。

Breeding and identification of Micromonospora purprua mutant CH20190225-107

In order to obtain high-yield Micromonospora purprua and improve the productivity and quality of GM. Methods The original strain GM20190109-15 is multiple mutated by ARTP& lithium chloride. Result We obtain a mutant strain CH20190225-107 by high throughput screening. The peak time of each component of the mutant strain is consistent with the contrast by high performance liquid chromatography. The potency is increased from 1547 U/mL to 2280 U/mL. The shake flask titer increased by 47.4% compared with the original strain, the relative proportions of component C1, C1a, C2a, and C2 are 29%, 23%, 25%, and 23% respectively, as well as stable inheritable. Conclusion In this study, it is effective for improving the yield and quality of gentamicin by the combined mutagenesis of ARTP and lithium