Protective effects of sciadopitysin against methylglyoxal-induced degeneration in neuronal SK-N-MC cells |
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| Authors: | Kwang Sik Suh Suk Chon Woon Won Jung Eun Mi Choi |
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| Affiliation: | 1. Department of Endocrinology and Metabolism, Kyung Hee University Hospital, Seoul, Republic of Korea;2. Department of Endocrinology and Metabolism, Kyung Hee University Hospital, Seoul, Republic of Korea
Department of Endocrinology and Metabolism, Kyung Hee University School of Medicine, Seoul, Republic of Korea;3. Department of Biomedical Laboratory Science, College of Health Sciences, Cheongju University, Cheongju, Republic of Korea |
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| Abstract: | The accumulation of advanced glycation end products (AGEs) causes metabolic dysfunction and neuronal cell damage. Methylglyoxal (MG) is a major glycating agent that reacts with basic residues present in proteins and promotes the formation of AGEs. Sciadopitysin, a type of biflavonoid, exerts protective effects against neuronal cell damage; however, the underlying mechanisms have not been studied. This study aimed to investigate the mechanisms underlying the protective effects of sciadopitysin against MG-mediated cytotoxicity in SK-N-MC neuroblastoma cells. Our results demonstrated that pretreatment of SK-N-MC cells with sciadopitysin improved the cell viability that was inhibited by MG and inhibited the apoptosis induced by MG. Sciadopitysin attenuated intracellular Ca2+, NOX4 levels, oxidative stress, and MG-protein adduct levels, and increased nuclear Nrf2 and glyoxalase 1 levels in the presence of MG. These results suggest that sciadopitysin exerts neuroprotective effects against MG-induced death of human SK-N-MC cells via its antioxidative action. This study highlights sciadopitysin as a promising candidate for antioxidant therapy and designing natural drugs against AGE-induced neurodegenerative disorders. |
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| Keywords: | methylglyoxal oxidative stress sciadopitysin SK-N-MC cells |
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